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Use of fecal microbiota transplantation as an adjuvant to the post-surgical treatment of equines undergoing enterotomy of the large colon: partial results
de Matos Curvelo de Barros, Aline...
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Introduction
As the large colon enterotomy can lead to dysbiosis, the goals of this study are to evaluate the intestinal microbiota after surgery and to verify if the Fecal Microbiota Transplantation (FMT) helps in the return to eubiosis.
Materials And Methods
The experiment occurs in Brazil, with 20 horses divided into two groups, control and treatment. The FMT was performed with feces of four healthy horses, 1.5Kg diluted in 2L of saline, 24h after surgery. Feces, collected in 4 moments (the first from the colon), were subjected to bacterial DNA extraction and amplification of the V4 region of the 16SrRNA gene. Alpha and beta diversity were analyzed, and statistical analysis by Kruskall-Wallis and PERMANOVA tests.
Results
At this time, six horses controls have been evaluated and four in the treatment. When collection moments are not considered there was a difference in alpha diversity between the total samples collected from the control and donors (p=0.003868), and in beta diversity (p=0.034). It was inferred that at the phylum level there was an increase in Proteobacteria after surgery regardless of the group with a decrease at the last moment.
Discussion And Conclusion
Results are partial and it is still not possible to verify whether FMT helps in the return to eubiosis, but the increase in Proteobacteria in the postoperative in both groups suggests dysbiosis. Reduction of this phylum at the last moment of collection suggests a recovery or adaptation of the microbiota. The differences in alpha and beta diversity between donors and controls consolidate the knowledge that there is a difference in the microbiota between healthy and animals subjected to intestinal insult. It is concluded that the microbiota probably undergoes changes due to colic and/or surgery, and that changes in bacterial phyla may be related to the adaptations to return to eubiosis.
References
- Arnold CE; Pilla, R. What Is the Microbiota and What Is Its Role in Colic? Veterinary Clinics: Equine Practice; 2012.
- Di Pietro R; Arroyo LG; Mathilde, L; Costa, M. Effects of concentrated fecal microbiota transplant on the equine fecal microbiota after antibiotic-induced dysbiosis. Canadian Journal of Veterinary Research 2023; 10 (4):85-96.
- Tuniyazi M; Wenqing W; Naisheng Z. Systematic Review of Current Applications of Fecal Microbiota Transplantation in Horses. Veterinary Sciences 2023; 10 (4):290.
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About
Affiliation of the authors at the time of publication
1 University of São Paulo - School of Veterinary Medicine and Animal Science, Internal Medicine, São Paulo, Brasil ; 2 University of São Paulo - Institute of Biomedical Sciences, Immunology, São Paulo, Brasil
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