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Concentrations of crosslinked C-telopeptide fragments of type II collagen after serial administration of allogeneic stem cells derived from synovial fluid in equine osteoarthritis model
Pereira Rodrigues, Nubia Nayara...
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Introduction: Several studies have demonstrated beneficial effects of the application of mesenchymal stem cells (MSCs) in osteoarthritis (OA) and many biomarkers are utilized for monitoring the degeneration of cartilage. Hence, crosslinked c-telopeptide fragments of type II collagen (CTX-II) showed consistent correlation with OA. The aim of this study is to monitor the CTX-II levels in the synovial fluid (SF) of surgically OA induced joints treated with three intra-articular applications of allogeneic MSCs derived from SF.
Materials And Methods: Twelve animals with experimentally induced OA were randomized to receive intra-articular applications of allogeneic MSCs (three doses of 107 MSCs administered at day 0, day 15 and day 45) in the treated group (TG). The control group (CG) received three applications of Ringer’s lactate solution at the same time points. The study was continued for another 135 days, with animals evaluated at six different times. Samples of SF collected were analyzed for CTX-II by ELISA.
Results: During an observation period of 175 days post joint injury, both groups had the same mean CTX-II (568 pg/ml - mean diff. - 0.06) in SF before treatment or sham treatment. However, after the first treatment the concentrations were lower in the TG at all time points, but significantly lower at the last time point (P=0.04).
Discussion/Conclusions: Several studies have shown that cartilage degradation increases as OA progresses, and monitoring CTX-II levels can provide insight into the extent of cartilage degradation and help assess disease progression.Throughout the study, TG had lower CTX-II levels, although only at the final time point was statistically significant. Our results suggest that treatment with three intra-articular applications of allogeneic MSCs was able to slow the degradation of type II collagen, which is a major component of cartilage. Further studies should investigate the potential of MSCs for reducing cartilage degradation.
References
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- Broeckx SY, Seys B, Suls M, Vandenberghe A, Mariën T, Adriaensen E, et al. Equine Allogeneic Chondrogenic Induced Mesenchymal Stem Cells Are an Effective Treatment for Degenerative Joint Disease in Horses. Stem Cells Dev. 2019 Mar 15;28(6):410–22.
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Affiliation of the authors at the time of publication
university Of Sao Paulo, Vcm, , Brasil ; 2 university Of Sao Paulo, Vci, São Paulo, Brasil ; 3 university Of Sao Paulo, Vcm, São Paulo, Brasil
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