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Chondroitin sulfate urinary excretion following chondroitin sulfate and glucosamine oral supplementation in horses with induced osteoarthritis
Pereira Rodrigues, Nubia Nayara...
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Introduction: Progression of osteoarthritis can be assessed through monitoring changes in urinary chondroitin sulfate (CS) levels. The increase in excretion may be related to a higher turnover rate of the extracellular matrix, due to increased chondral metabolic activity.This study investigated urinary CS excretion in horses supplemented with CS and glucosamine (GlcN).
Materials And Methods: Metacarpophalangeal osteoarthritis was surgically induced in sixteen Warmbloods. The horses were divided into a control group (CG) and treated group (TG). The TG received a 10g oral supplement (1,9g CS; 4g GlcN) twice daily, starting from the day of the OA induction, and continued for more than 120 days. Urine samples were collected by spontaneous urination at 9 predetermined time points. CS concentration was identified by 0.5% agarose gel electrophoresis in 1,3-diaminopropane acetate buffer followed by densitometry. Creatinine (Crea) concentration was used as a correction factor for CS excretion.
Results: Despite addition of exogenous CS, decreases in the rate of urinary excretion of CS was observed at some times in TG (P=0.03), which did not occur in the CG. However, when the rate is corrected for urinary creatinine (g), the TG animals presented no differences in CS/Crea ratio between time points. Animals treated with oral CS and glucosamine had urinary CS/Crea excretion similar to the control group (P=0.08).
Discussion/Conclusions: Exogenous CS supplementation didn’t significantly change the urinary CS/ Crea excretion rate between groups. Although orally administered CS and GlcN are absorbed, the bioavailability may be variable and consequently influence the excretion rate. The increase in CS excretion may be related to a higher turnover rate of the extracellular matrix in horses, due to a high metabolic chondral activity. Based on the doses used in this study after 120 days with CS and GlcN did not significantly affect CS excretion in horse urine.
References
- Baccarin RYA, Machado TSL, Lopes-Moraes AP, Vieira FAC, Michelacci YM. Urinary glycosaminoglycans in horse osteoarthritis. Effects of chondroitin sulfate and glucosamine. Res Vet Sci. 2012 Aug;93(1):88–96.
- Contino EK. Management and Rehabilitation of Joint Disease in Sport Horses. Vol. 34, Veterinary Clinics of North America - Equine Practice. W.B. Saunders; 2018. p. 345–58.
- Fiorin V, Rodrigues NNP, Cota LO, Seidel SRT, Barbosa P, Tokawa PKA, et al. Clinical study of chondroitin sulfate urinary excretion following intramuscular application of the chondroitin sulfate and glucosamine association in horses. Arq Bras Med Vet Zootec. 2023;75(2):355–9.
- Michelacci YM, Glashan RQ, Schor N. Urinary excretion of glycosaminoglycans in normal and stone forming subjects. Vol. 36, Kidney International. 1989.
- ira FAC, Baccarin RYA, Aguiar JAK, Michelacci YM. Urinary Excretion of Glycosaminoglycans in Horses: Changes With Age, Training, and Osteoarthritis. J Equine Vet Sci. 2005;25(9):384–6.
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Affiliation of the authors at the time of publication
1university Of Sao Paulo, Vcm, São Paulo, Brasil ; 2university Of Sao Paulo, Vci, São Paulo, Brasil.
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