Get access to all handy features included in the IVIS website
- Get unlimited access to books, proceedings and journals.
- Get access to a global catalogue of meetings, on-site and online courses, webinars and educational videos.
- Bookmark your favorite articles in My Library for future reading.
- Save future meetings and courses in My Calendar and My e-Learning.
- Ask authors questions and read what others have to say.
Effect of a topical anaesthetic formulation on viral load in lambs naturally infected with orf virus
Lacasta, D.; Reina R.; Ramos, J.J...
Get access to all handy features included in the IVIS website
- Get unlimited access to books, proceedings and journals.
- Get access to a global catalogue of meetings, on-site and online courses, webinars and educational videos.
- Bookmark your favorite articles in My Library for future reading.
- Save future meetings and courses in My Calendar and My e-Learning.
- Ask authors questions and read what others have to say.
Read
Objectives: Orf is a highly contagious eruptive skin condition of sheep and goats, caused by a Parapoxvirus with a worldwide distribution. It affects mainly lambs and kids, with more serious outbreaks often associated with intensive hus- bandry, causing significant financial losses to livestock production. It is also a zoonotic disease, affecting mainly people via direct or indirect contact with infected animals. Vaccination remains the preferred option to control the disease. However, currently in Spain and many other countries, no orf vaccine is available. The treatment of this disorder referred to as Conta- gious Ecthyma and Scabby Mouth, involves standard hygiene practices and management of presumptive secondary infections.
The wound dressing formulation, Tri-Solfen® (Animal Ethics Pty Ltd, Australia) is registered for use in sheep in Australia, cattle in Australia, New Zealand and Laos, and is in the process of being registered to be used in cattle and pigs in Europe and other countries. This formulation offers numerous advantages over current therapies, particularly as it has been confirmed as providing pain relief and more rapid healing of lesions (Windsor et al, 2016). The formulation contains two local anaesthetics (lignocaine and bupivacaine), adrenalin and an antiseptic (cetramide) in a gel formulation that creates a barrier effect with a pH of ~2.7. This numbs the pain of lesions, reduces their infectivity, hastens healing, potentially reduces weight loss, and the antisepsis properties avoids the need for other treatments, including antibiotics.
This study explores potential antiviral roles and healing properties of Tri-Solfen® in orf naturally infected lambs through viral genome real time PCR quantification and tissue culture in ovine primary cells.
Materials and methods: This study was approved by the Ethical Commission of the University of Zaragoza. Fourteen one-month-old Rasa Aragonesa lambs, naturally infected with orf, were recruited from a farm where an outbreak of orf disease was occurring. The animals were selected at the early stages of the infection when lesions were initially advised and divided into two cohorts: Group A (n=11) consisting of animals with orf lesions treated with Tri-Solfen® and Group B (n=3), a control group without treatment.
Cotton swabs were obtained before treatment (T0) and days 1 (T1), 3 (T2) and 5 (T3) post-treatment, then submitted to direct DNA extraction and real-time PCR quantification (Ex- opol) or to incubation with primary tissue cultures from ovine skin fibroblasts (OSF) and T-immortalized goat embryonic fibroblasts (TIGEF). Orf virus quantification was performed by real time PCR on DNA from cultured cells at day 0 and 5 post-treatment. Data were analyzed using the non-parametric Wilcoxon test for paired samples and by T-Student’s test for unrelated samples.
Results: In the study carried out using quantitative PCR, no significant differences were found between day 0 pre-treatment (T0) and day 5 post-treatment (T3) (p=0.722). However, when the viral load was assessed in primary tissue cultures of ovine skin fibroblasts (OSF) and T-immortalized goat embryonic fibroblasts (TIGEF), there was a reduction in both groups between T0 and T3 that was significant in the OSF cell cultures (p<0.05).
Conclusions: These results suggest that despite the presence of the viral DNA in the orf lesions at 5 days post-treatment, this may belong to inactivated virus as the viral load obtained after cell culture of the samples of the treated animals was significantly less than that obtained from controls. These findings suggest that as treatment of orf lesions with Tri- Solfen® reduces the viral load present in lesions, such therapy may also alter the clinical progression and transmission in outbreaks of Contagious Ecthyma.
Keywords: Sheep, orf virus, topical anaesthetic formulation, viral load.
Get access to all handy features included in the IVIS website
- Get unlimited access to books, proceedings and journals.
- Get access to a global catalogue of meetings, on-site and online courses, webinars and educational videos.
- Bookmark your favorite articles in My Library for future reading.
- Save future meetings and courses in My Calendar and My e-Learning.
- Ask authors questions and read what others have to say.
About
Affiliation of the authors at the time of publication
Veterinary Faculty of Zaragoza and IA2. University of Zaragoza, Zaragoza, Spain;
Institute of Agrobiotechnology (CSIC-Gobierno de Navarra), Mutilva, Spain;
Laboratorios EXOPOL, diagnóstico y autovacunas, San Mateo de Gállego, Spain;
Faculty of Veterinary Science. University of Sydney., Sydney, Australia.
Comments (0)
Ask the author
0 comments