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Comparison of bull semen motility using a portable semen analysis device and computer-assisted sperm analyser
Mitchell, K., Beggs, D., Mansell, P...
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Objective
Bull breeding soundness evaluations (BBSEs) in Australia include a physical exam, scrotal circumference measurement, crush-side semen motility evaluation and often remote assessment of sperm morphology to provide a comprehensive assessment of a bulls’ fertility and risk. The crush-side motility component of the examination typically involves the use of a microscope with a heated stage, requiring a large amount of space and power. The semen is assessed for progressive motility and an estimate is provided based on the forward movement of sperm observed by the practitioner. The crush-side assessment of sperm motility is relatively subjective and can be influenced by the experience of the practitioner, environmental temperature, concentration of the semen and the volume assessed. Previously developed for swine, the use of iPad-based sperm motility analysers has been developed to include most species of production animals including cattle.
The objective of this study was to compare the motility of fresh bull semen using a portable iPad-based semen motility analyser (iSperm) and a computer assisted sperm analyser (Androvision - Minitube).
Material & Methods
Semen was collected from three different proven stud bulls at a commercial bull collection facility, extended using Andromed (Minitube) extender and chilled. The ejaculates were transported to the semen laboratory the following day for assessment using the iSperm and Androvision system. The ejaculates were diluted and assessed simultaneously by the Androvision and iSperm for motility and concentration. The motility of the ejaculates was altered using additions of flash frozen dead sperm to allow for a greater scope of motilities to be assessed by the two devices. The motility, progressive motility and concentration were recorded. Analysis was repeated ten times to allow for later calculation of a coefficient of variation.
Results
A full analysis of the data is yet to be obtained however, the raw data indicates there is a repeatable correlation between the Androvision and iSperm when assessing progressive motility & concentration of fresh bull ejaculates.
Conclusion
Similar studies in other species have validated the use of a portable semen motility assessment device such as iSperm, allowing for assessment of semen in the field and removing assessor bias and environmental factors.
Keywords: bull, fertility, semen analysis
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