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Evaluating the correlation of digital dermatitis infection and genetic variants to predict gene resistance in lactating Holstein cattle
Barbara Jones and Grace Canny
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Objective
Digital Dermatitis (DD) is a contagious bacterial hoof infection. Discovery of genetic resistance to DD will allow dairy producers to select for this trait, therefore reducing lameness caused by DD infections. This study evaluated the correlation of DD infection and genetic variants to predict genetic resistance to DD in Holstein cattle on a commercial dairy farm in central Texas.
Materials and methods
Lactating dairy cattle were scored on a 0 (no lesions present) to 4 (chronic lesions) scale. Blood samples were collected on lactating cows (n = 45) from the coccygeal vein. Cows were identified as having a lesion (n = 35) and not having a lesion (n = 10). Genomic DNA was isolated from 200 uL of whole blood, Genomic DNA was purified from whole blood samples from (n = 45) lactating cows. Four previously reported SNPs were evaluated to determine correlation to DD resistance by qPCR melt curve analysis. SNPs in the 3’UTR of Interleukin-8 (IL8) and in exon 3 of Toll-Like Receptor 4 (TLR4) were analyzed by Chi-Square in SAS (Version 9.4 SAS Institute, Inc., Cary, NC) to determine the frequencies of different genotypes present amongst the resistant and affected animals. [...]
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