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OPU and ICSI in the horse
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Key information
Event Information
Due to the great success of our 2 courses during the last years we have been asked by various vets, to set up another one.
Well, here you go. The program has been updated with new topics covering the state of the art in opu and ICSI science in horses.
We are happy to have well worldwide known experts in their field of expertise from Denmark, The Netherlands, the United States, and Australia.
This course addresses the participants interrested to gain deeper profound knowledge of the whole OPU and ICSI procedure.
For the first time instead of doing just demonstrations, we will provide the opportunity for the participants to practice themselves and as one highlight, there will be three real ICSI sessions on the last day on oocytes recovered the previous day.
The registration fee includes 2 nights' accommodation, full board including 3 lunches, coffee breaks, 2 dinners, printed proceedings, and access to the wellness facilities of the Hotel.
If you require additional nights @122 Euro incl. breakfast, please book them with the registration as well
Agendas
29 Sept. 2023 Day 1
07:45 - Registration and Welcome
08:00 - Introduction in advanced reproductive technologies in horses. History and future (KH)
09:00 - Selection and preparation of the appropriate recipient mare, suitable stallion
09:45 - Coffee break
10:00 - The first 10 days of an equine embryo: from a fertilized oocyte to blastocyst. - Are there
differences in ICSI embryo development. With time lapse videos
11:00 - Equine embryo grading
11:30 - From oocyte to transferable blastocyst: oocyte recovery, searching oocytes, handling fresh oocytes, evaluation, washing, transport, maturation, ICSI
13:00 - Lunch
14:00 - Wet labs (participants will be divided into 3 groups):
- Material & Methods used for OPU / oocyte handling, shipping / ICSI / ET
- Straw thawing, cutting, refreezing, and selection of the best spermatocyte for ICSI
- Practical OPU training on dead ovaries, including set up of the whole system
16:00 - Wetlabs as above with change of groups
18:00 - Wetlabs as above with change of groups
20:00 - End of day 1, transfer to hotel
20:30 - Gala Dinner
30 Sept. 2023 Day 2
08:00 - Sperm selection for ICSI using micro fluids
08:30 - Non-invasive assessent of oocyte developmental competence
09:15 - Selection and preparation of the appropriate donor mare (Jannie Spanner), suitable stallions (Hans Hurkmans), and recipient mare (Jannie Spanner).
10:00 - Management of the recipient mare: Differences in pregnancy rates,
early embryo loss, twins, triplets, monitoring of foaling
10:30 - Thawing and transfer of ICSI embryos
11:00 - The terminal mare
11:15 - Coffee break
12:00 - Ovary recovery in a euthanized horse - preparation & shipment
13:00 - Lunch
14:00 - Wet-labs: (participants divided into 3 groups):
- OPU session on a horse, oocyte searching and preparation.
- Freezing, vitrification, packaging of embryos
- Embryo thawing and evaluation
16:00 - Wet-labs (participants divided into 3 groups)
18:00 - Wet-labs (participants divided into 3 groups)
20:00 - End of day 2
20:15 - BBQ Party
1 Oct. 2023 Day 3
08:00 - Freezing equine embryos and cryopreservation
08:45 - Actual overview on OPU sessions, labs, blastocyst rates, foal rates
09:30 - How to set up an OPU/ICSI Lab: two cases (Investment, ROI, marketing)
10:30 - Wetlabs (participants divided in 3 groups)
- ICSI session with oocytes obtained at the previous day
- Practise on embryotransfer, standard vs Whilser
- Scraping ovaries
12:30 - Lunch
13.30 - Wetlabs as above
15:30 - Wetlabs as above
17:30 - End of course, final discussion
Speakers
- Hans Hurkmans, ET/OPU Specialist / General manager, Hurkmans ET B.V.
- Jannie Spanner, CertAVP Equine Stud Medicine. Jannie is partner of vetempryo APS located in Röedekro Denmark
- Kylie Dunning, Ext-Funded Research Fellow (C), University of Adelaide
- Pouya Dini, Assistant Professor Health & Reproduction, Chief of the Equine Reproduction Service, School of Veterinary Medicine University of California, Davis