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A New High Sensitive Keratan Sulfate Biomarker to Detect Metabolic Changes of Cartilage in Horses
M. Okumura, V. Lettry, K. Mitsuda...
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Introduction: Early detection of degenerated articular cartilage is somewhat limited. Initial changes cannot be detected by conventional diagnostic devises. Enzyme-linked immunosorbant assay (ELISA) by anti-KS antibody 1/20/5D4, suggested its usefulness as a marker of osteoarthritis, and it has been studied ever since. The purpose of the present study was to evaluate two different anti-KS monoclonal antibodies, 5D4 (KS5D4) as a clinical diagnostic tool to detect cartilage metabolism in horses. Materials and Methods: Serum samples were corrected from 30 horses free of lameness in a horse breeding farm in Hokkaido, Japan, and analyzed KS5D4 and HSKS, using enzyme-linked immunosorbant assay (ELISA: KS5D4; c/n280565, HSKS: c/n280567; Seikagaku Corp., Tokyo, Japan). To compare the effects of the age and the exercise group, as well as their interaction on serum levels of each of the biomarkers, using a ANOVA measure. P values less than 0.05 were considered significant. Results: KS5D4 and HSKS concentration were valued for all the animals in the corresponding age and physical activity groups did not show any correlation. KS5D4 presented slight variation yet they were not significantly different in any stage of physical activity or age, when analyzed with KS5D4 antibody. From HSKS, it could be observed that keratan sulfate concentration holds a tendency to be reduced as the horse as it matures. Discussion and conclusion: Correlation could not be found between KS5D4 and HSKS. Measured KS values by HSKS were possible and more sensitively indicating a consequence of cartilage turn over rate held by younger animals. It is possible to assume that screening HSKS would be of more clinical value to identify cartilage lesions at the joint in horses.
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