Get access to all handy features included in the IVIS website
- Get unlimited access to books, proceedings and journals.
- Get access to a global catalogue of meetings, on-site and online courses, webinars and educational videos.
- Bookmark your favorite articles in My Library for future reading.
- Save future meetings and courses in My Calendar and My e-Learning.
- Ask authors questions and read what others have to say.
Diarrhea in Buffalo Calves in Minas Gerais, Brazil: The Involvement of Enterobacteria
De Oliveira Henrique Sousa, C...
Get access to all handy features included in the IVIS website
- Get unlimited access to books, proceedings and journals.
- Get access to a global catalogue of meetings, on-site and online courses, webinars and educational videos.
- Bookmark your favorite articles in My Library for future reading.
- Save future meetings and courses in My Calendar and My e-Learning.
- Ask authors questions and read what others have to say.
Read
Objectives: the aim of this study was to investigate the involvement of pathogenic escherichia coli and salmonella spp. in the etiology of diarrhea in buffalo calves up to 3 months old.
Materials and Methods: one hundred fifty-two fecal samples were collected from diarrheic and non-diarrheic calves aged one to 90 days from five dairy farms in the state of Minas gerais, Brazil. Fecal samples were inoculated into buffered peptone water and incubated. the cultures were then plated onto Macconkey agar plates and inoculated into tetrathionate Broth. the cultures from tetrathionate Broth were plated onto Hektoen and X lt4 media plates and incubated. A multiplex Pcr was performed to determine the presence of virulence factors of escherichia coli strains, which were classified into four pathotypes according to the Results: enterotoxigenic e. coli ( ETEC ), enteropathogenic e. coli ( ePec ), enterohemorrhagic e. coli ( eHec ) and shiga toxin-producing e. coli ( STEC ). colonies of salmonella spp. were tested by Pcr for genus confirmation. calves were divided into three groups according to their age: animals aged one to 30 days (F1), 31 to 60 days (F2) and 61 to 90 days (F3).
Results: of the 152 calves, 107 (70,4%) had diarrhea and 45 (29,6%) were asymptomatic. the frequency of diarrhea within each age group was 86%, 56% and 50% for F1, F2 and F3, respectively. e. coli pathotypes identified were ePec (eae+), STEC ( stx1+ and/or stx2+), eHec (eae/ stx1 and/or stx2+) and ETEC ( k99/F41/ sta+). among animals with diarrhea, 5,6% (6/107) had eHec isolated from feces, 13,1% (14/107) stec, 4,7% (5/107) ePec and 0,9% (1/107) etec. the frequency among feces from non-diarrheic animals was 8,9% (4/45) for eHec, 13,3% (6/45) for stec, 6,7% (3/45) for ePec and ETEC wasn’t identified. salmonella spp. was isolated from feces of three animals, of which two had diarrhea, one had concomitant infection with STEC and were in F1 and F2 groups. the third animal was asymptomatic and belonged to F1 group.
Conclusions: the presence of pathogenic e. coli and salmonella spp. in feces of buffalo calves demonstrate that these agents may be involved in diarrhea of these animals and indicate that, similarly to cattle, buffaloes may also be a source of bacterial infection for humans.
Get access to all handy features included in the IVIS website
- Get unlimited access to books, proceedings and journals.
- Get access to a global catalogue of meetings, on-site and online courses, webinars and educational videos.
- Bookmark your favorite articles in My Library for future reading.
- Save future meetings and courses in My Calendar and My e-Learning.
- Ask authors questions and read what others have to say.
About
Affiliation of the authors at the time of publication
Departamento de Medicina Veterinária Preventiva of the Escola de Veterinária of the Univerisidade Federal de Minas Gerais, Brazil
Comments (0)
Ask the author
0 comments