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Investigation of in vitro Conditions Required for Biofilm Formation in Escherichia coli Isolated from Mare Reproductive Tract

Author(s):
Paccamonti D.L.,
Lyle S.K.,
Krekeler N. and
Beehan D.P.
In: SFT - Theriogenology Annual Conference - San Antonio, 2015 by Society for Theriogenology
Updated:
AUG 09, 2015
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Biofilm formation has been suggested to be important for chronic bacterial endometritis of mares. The crystal violet (CV) assay is used commonly in human medicine to evaluate the biofilm forming potential of bacterial isolates. The CV assay evaluates the early steps in biofilm formation, i.e. bacterial attachment and extracellular polymatrix (EPS) production. The CV dye binds to both cells and EPS, and it is a measure of total biofilm biomass formed. However the CV assay is not standardized across biomedical disciplines, as published methodologies vary considerably among laboratories. The aim of this project was to evaluate the effects of two incubation conditions (time and orbiting) on the biofilm forming potential of Escherichia coli (E.coli) isolates from the mare reproductive tract. We hypothesized that different methods of incubation affect biofilm development. [...]

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How to reference this publication (Harvard system)?

Paccamonti, D. et al. (2015) “Investigation of in vitro Conditions Required for Biofilm Formation in Escherichia coli Isolated from Mare Reproductive Tract”, SFT - Theriogenology Annual Conference - San Antonio, 2015. Available at: https://www.ivis.org/library/sft/sft-theriogenology-annual-conference-san-antonio-2015/investigation-of-vitro-conditions-required-for-biofilm-formation-escherichia-coli-isolated-from-mare (Accessed: 24 September 2023).

Author(s)

  • Paccamonti D.L.

    DVM MS
    Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University
    Read more about this author

  • Lyle S.K.

    DVM MS
    Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University
    Read more about this author
  • N. Krekeler

    Krekeler N.

    DVM, PhD, DACT, DrMedVet
    Faculty of Veterinary and Agricultural Sciences, University of Melbourne
    Read more about this author
  • David Beehan

    Beehan D.P.

    MVB MS PhD DipACT
    Department of Veterinary Clinical Sciences, Louisiana State University
    Read more about this author

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The Society for Theriogenology is an organization of veterinarians dedicated to animal reproduction, whose mission is to promote standards of excellence in reproductive medicine, to provide outreach and education to veterinarians, and to foster continual improvements in theriogenology.

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