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Evaluation of a Novel Swim-Up Protocol and Protein Source on Canine Sperm Progressive Motility, Concentration and Acrosome Reaction
Author(s):
A. Lengele, L. Gentle, A. Burns and...
Updated:
AUG 09, 2015
Languages:
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Introduction
Swim-up has been considered a preferred method for separating non-motile sperm in an ejaculate. However, the long incubation time and equipment necessary for swim-up make this process clinically impractical. Our laboratory hypothesized that these problems could be avoided for canine applications with a shorter incubation time at room temperature (RT). The objective was to compare the standard swim-up method (STAN) to this novel method (NOVEL). In addition, we hypothesized that incubation with heat-treated canine serum (HTCS) would improve swim-up results compared to bovine serum albumin (BSA) or fetal calf serum (FCS). [...]
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