The Detection of Tritrichomonas Foetus in Bovine Semen with Centrifugation and PCR

Bovine trichomoniasis caused by Tritrichomonas foetus, is a true venereal disease of cattle that is spread only through coitus. Diagnostic techniques have recently improved with the emergence of a more sensitive and specific polymerase chain reaction (PCR) techniques. This is an improvement from traditional culture methods, but better diagnostic and collection methods are still needed given the serious consequences of inaccurate diagnosis. The objective of the study was to determine if a T. foetus infection could be detected using PCR in pre-seminal fluid and a seminal sample collected from known positive bulls. [...]