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Effect of platelet rich plasma lysate and fibroblast growth factor 2 on sperm motility in stallions
Fabio Pinaffi, Robyn Wilborn...
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Semen extenders are continually tested to improve sperm motility, longevity, and, consequently, improve fertility. Growth factors (GFs) modulate cell function, which could be advantageous to sperm by improving motility. In humans and mice, fibroblast growth factor 2 (FGF2) improved sperm motility. Platelet rich plasma (PRP), which is rich in growth factors (GFs) including FGF2, reduced postmating inflammatory response within the uterus when infused 24 hours before or after artificial insemination. Effect of PRP on sperm is not determined. Objective was to evaluate the effect of adding either pooled PRP lysate (PRPL) or recombinant equine FGF2 (reFGF2) at varying concentrations to semen extended in commercial equine semen extender (INRA 96). PRP lysate was used instead of PRP, since it is more purified and highly concentrated in GFs. Eight treatments were tested using concentrations of 1, 2.5, 5, and 10% of PRPL containing 1 IU/ml of heparin, and 0.1, 1, 10, and 100 ng/ml of reFGF2 and compared to control groups with and without 1 IU/ml of heparin. Heparin use was based on a previous titration to prevent gel formation from PRPL reaction with semen extender, precluding motility analysis. Motility parameters were evaluated with samples standardized to 50 million sperm/ml using computer assisted semen analysis (CASA) at hours 0, 0.5, 1, 1.5, 6, and 24 after treatment. For both PRPL and reFGF2 treatments, there were no differences (p = 0.99) in motility among groups at any time point, with ranges from 66 to 60% at hour 0 and 46 to 49% at hour 24. Interestingly, results concentrations of PRPL > 5% induced sperm agglutination via head-to-head attachment (HHA), starting at hour 1, whereas PRPL at concentrations below 2.5% did not induce HHA nor affect sperm motility. In addition, HHA was objectively detected by decreases in total number of cells counted per field (total cells) and estimated concentration measured by CASA, assuming that only free sperm were counted, whereas sperm entrapped by HHA not counted. Decreases in total sperm and concentration were different (p < 0.0001) for 5 and 10% PRP groups, being more pronounced for 10% PRPL group, suggesting a dose-dependent characteristic of HHA induced by PRPL. One important finding was that PRPL did not kill sperm, besides inducing HHA. Although no motility improvement was observed, present results suggests that direct addition of PRPL in semen extender at doses below 5% could be implemented to reduce postmating uterine inflammatory response, without substantially affecting sperm motility. However, further research on uterine inflammatory response is needed to test this hypothesis.
Keywords: Growth factors, sperm motility, endometritis, head-to-head attachment
This manuscript was originally published in the journal Clinical Theriogenology Vol 12(3) Sept 2020. Clinical Theriogenology is the official journal of the Society for Theriogenology (SFT) and the American College of Theriogenologists (ACT). This content has been reproduced on the IVIS website with the explicit permission of the SFT/ACT.
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Affiliation of the authors at the time of publication
Department of Clinical Sciences, Auburn University, Auburn, AL
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