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Acupuncture reduces milk N-acetyl beta-D-glucosaminidase in dairy cows with mastitis
Elise Ryan, a. Michelle Kutzler b
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Acupuncture (AP) is used by dairy veterinarians as a complementary treatment for various conditions. 1 NAGase is a lysosomal glycosidase released from mammary epithelial cells during mastitis.2 Objective was to determine if AP reduces mammary inflammation. We hypothesized that dairy cattle with subclinical mastitis treated with an intramammary antibiotic and AP would have lower somatic cell count (SCC) and milk N-acetyl beta-D-glucosaminidase (NAGase) concentrations compared to cattle treated with only an intramammary antibiotic. Lactating dairy cows at Oregon State University Dairy Research Center were selected (cows with SCC score of SCC > 500,000 cells/ml) based on Dairy Herd Improvement Association reports. A California Mastitis Test (CMT) determined the quarter that had high SCC (CMT score > 1). Pretreatment milk samples (pre) were collected for SCC scoring and NAGase concentrations. SCC was measured by DeLaval cell counter (Tumba, Sweden) and NAGase concentrations were determined by an ELISA kit (#MBS090625, MyBioSource, San Diego, CA). Cows were treated with an intramammary infusion of ceftiofur hydrochloride (Spectromast LC® , Zoetis, Parsippany, NJ) and randomly assigned to AP group (n = 7) and nonAP (control) group (n = 7). Both group cows were restrained for 30 minutes in a head catch 4 times, 12 hours apart. Six AP points were used (bladder [BL] 30, BL 30-1, BL 49, kidney [KI] 10, conception vessel [CV] 2, CV 3). Posttreatment milk samples (post) were collected 14 days after presample collection. Pre CMT scores, NAGase concentrations, and SCC were compared to post scores within and between groups. Significance was defined as p < 0.05. Both control and AP groups had reduced post CMT scores (pre: 1.41 ± 0.49; post:
0.42 ± 0.66; p = 0.01 and pre: 1.21 ± 0.39; post: 0.57 ± 0.79; p = 0.039, respectively) but there was no
significant change in SCC in either group. In AP group but not in control group, NAGase concentrations were reduced (pre: 19.70 ± 3.65 U/L; post: 16.70 ± 2.05 U/L; p = 0.04 and pre: 25.60 ± 12.90 U/L; post: 20.40 ± 6.04 U/L; p = 0.20, respectively). Reduction in NAGase provided evidence for AP as a complementary treatment.
Keywords: Complementary treatment, California mastitis test, somatic cell count, subclinical
Acknowledgement Authors thank Oregon Dairy Farmers Association and Oregon State University’s E. R. Jackman Friends and Alumni and Continuing Researchers Program for funding.
References
- Hiebel K, Gentle L, Menino A, et al. Acupuncture prevents the postpartum reduction in matrix metalloproteinase type2 immunoexpression, tissue concentration and enzyme activity in bovine caruncles. Reprod Domest Anim 2019;54:1010-1017.
- Kitchen BJ, Middleton G, Salmon M. Bovine milk N-acetyl B-D-glucosaminidase and its significance in the detection of abnormal udder secretions. J Dairy Res 1978;45:15-20.
This manuscript was originally published in the journal Clinical Theriogenology Vol 12(3) Sept 2020. Clinical Theriogenology is the official journal of the Society for Theriogenology (SFT) and the American College of Theriogenologists (ACT). This content has been reproduced on the IVIS website with the explicit permission of the SFT/ACT.
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Affiliation of the authors at the time of publication
a Department of Integrative Biology,
b Department of Animal and Rangeland Sciences Oregon State University, Corvallis, OR
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