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Laboratory use in pregnancy loss diagnosis
Andrés de la Concha-Bermejillo...
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Pregnancy loss and perinatal mortality are important economic and public health issues for the livestock industry. To minimize losses and prevent human infections, rapid and accurate identification of the cause is critical. Veterinary diagnostic laboratories not only have an important role in identifying causes of pregnancy loss but also in monitoring (surveillance) infectious diseases that cause reproductive failure. Appropriate specimen collection and handling are essential prerequisites for reliable test results. Consequently, laboratory test procedures and their value may be compromised by using specimens that are not collected, labelled, handled or stored properly prior to testing. Principles of biosecurity and biocontainment must be strictly followed during sample collection. For accurate determination of the cause of pregnancy loss in ruminants, it is recommended to use a battery of diagnostic tests done in parallel. These tests include histopathology, real time polymerase chain reaction (rtPCR) assay, bacterial and viral isolation, detection of fetal antibodies in serum or fluid from thorax or abdomen, and maternal serology. Veterinary laboratory professional staff can provide consultation services to clients regarding test selection and testing strategies, interpretation of results, surveillance, disease prevention and control programs. This review provides essential information on collection of diagnostic samples and interpretation of test results for the diagnosis of most common infectious causes of pregnancy loss in ruminants. ...
Ruminants, abortion, diagnosis, pregnancy loss, laboratory
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