Endometrial Gene Expression of Inflammatory Cytokines and Serum Amyloid a in Mares Resistant or Susceptible to Persistent Endometritis

The objective of the study was to evaluate the endometrial gene expression of inflammatory cytokines (IL-1β, IL-1RA, IL-6, IL-8, IL-10, TNFα) and serum amyloid A (SAA) in response to inoculation of 105 CFU Escherichia coli in mares resistant or susceptible to persistent endometritis, respectively.

Mares were classified as resistant or susceptible based on their clinical response to uterine inflammation and endometrial quality. Endometrial biopsies were recovered 3, 12, 24 and 72 hours (h) post-inoculation. Relative gene expression analyses were performed by quantitative real-time PCR using SYBR green and specific primers. [...]