Skip to main content
menu sluit menu
Home Home
Login
Main navigation
  • Library
  • Calendar
  • e-Learning
  • News
    • Veterinary News In this section you find veterinary news
    • Recent Additions All content that was recently added to the IVIS library
  • Get involved
    • Donate Support IVIS, make a donation today
    • Media kit Promote your e-learning & events on IVIS
    • Add your e-learning & events to the IVIS calendar
    • Publish on IVIS Publish your work with us
  • About
    • Mission Our Mission Statement
    • What we do More info about IVIS and what we do
    • Who we are More info about the IVIS team
    • Authors See list of all IVIS authors and editors
  • Contact
User tools menu
User tools menu
Main navigation
  • Library
  • Calendar
  • e-Learning
  • News
    • Veterinary News In this section you find veterinary news
    • Recent Additions All content that was recently added to the IVIS library
  • Get involved
    • Donate Support IVIS, make a donation today
    • Media kit Promote your e-learning & events on IVIS
    • Add your e-learning & events to the IVIS calendar
    • Publish on IVIS Publish your work with us
  • About
    • Mission Our Mission Statement
    • What we do More info about IVIS and what we do
    • Who we are More info about the IVIS team
    • Authors See list of all IVIS authors and editors
  • Contact
Follow IVIS
  • Twitter
  • Facebook
Support IVIS

Breadcrumb

  1. Home
  2. Library
  3. Society for Theriogenology
  4. SFT - Theriogenology Annual Conference - Milwaukee, 2011
  5. Biopsy and Vitrification of Equine Expanded Blastocysts
Society for Theriogenology
Back to Table of Contents
Add to My Library
Close
Would you like to add this to your library?

Get access to all handy features included in the IVIS website

  • Get unlimited access to books, proceedings and journals.
  • Get access to a global catalogue of meetings, on-site and online courses, webinars and educational videos.
  • Bookmark your favorite articles in My Library for future reading.
  • Save future meetings and courses in My Calendar and My e-Learning.
  • Ask authors questions and read what others have to say.
Sign in Register
Comments
Share:
  • Facebook
  • LinkedIn
  • Mail
  • Twitter

Biopsy and Vitrification of Equine Expanded Blastocysts

Author(s):
Hinrichs K.
In: SFT - Theriogenology Annual Conference - Milwaukee, 2011 by Society for Theriogenology
Updated:
AUG 13, 2011
Languages:
  • EN
Back to Table of Contents
Add to My Library
Close
Would you like to add this to your library?

Get access to all handy features included in the IVIS website

  • Get unlimited access to books, proceedings and journals.
  • Get access to a global catalogue of meetings, on-site and online courses, webinars and educational videos.
  • Bookmark your favorite articles in My Library for future reading.
  • Save future meetings and courses in My Calendar and My e-Learning.
  • Ask authors questions and read what others have to say.
Sign in Register
SHARE:
  • Facebook
  • LinkedIn
  • Mail
  • Twitter
    Read

    Abstract

    Preimplantation genetic diagnosis and embryo cryopreservation have extensive clinical application in the horse. Biopsy of equine embryos is complicated by the presence of the equine embryonic capsule. Recently, we developed a technique to puncture the capsule and obtain cells from the trophoblast of expanded equine blastocysts, using micromanipulation with the Piezo drill. Biopsies were obtained without affecting the pregnancy rate of the blastocysts after transfer. Genetic analysis after whole genome amplification of the biopsied cells demonstrated some failure to detect signal, and allele dropout, and work is currently underway to improve the accuracy of genetic analysis. Observation that the blastocysts collapsed after biopsy, yet maintained viability, led to the hypothesis that the collapsed blastocyst might be successfully vitrified. Further work showed that vitrification of collapsed blastocysts using an ethylene glycol-containing medium was associated with high pregnancy rates after transfer (71% of embryos produced pregnancies that developed normally to the heartbeat stage), thus providing the first successful procedure for cryopreservation of expanded equine blastocysts. These advances in embryo manipulation techniques should find wide application in clinical equine practice. [...]

    View full text
    Back to Table of Contents
    Add to My Library
    Close
    Would you like to add this to your library?

    Get access to all handy features included in the IVIS website

    • Get unlimited access to books, proceedings and journals.
    • Get access to a global catalogue of meetings, on-site and online courses, webinars and educational videos.
    • Bookmark your favorite articles in My Library for future reading.
    • Save future meetings and courses in My Calendar and My e-Learning.
    • Ask authors questions and read what others have to say.
    Sign in Register
    Comments (0)

    Ask the author

    0 comments
    Submit
    Close
    Would to like to further discuss this item?

    Get access to all handy features included in the IVIS website

    • Get unlimited access to books, proceedings and journals.
    • Get access to a global catalogue of meetings, on-site and online courses, webinars and educational videos.
    • Bookmark your favorite articles in My Library for future reading.
    • Save future meetings and courses in My Calendar and My e-Learning.
    • Ask authors questions and read what others have to say.
    Sign in Register
    About

    How to reference this publication (Harvard system)?

    Hinrichs, K. (2011) “Biopsy and Vitrification of Equine Expanded Blastocysts”, SFT - Theriogenology Annual Conference - Milwaukee, 2011. Available at: https://www.ivis.org/library/sft/sft-theriogenology-annual-conference-milwaukee-2011/biopsy-and-vitrification-of-equine-expanded-blastocysts (Accessed: 08 June 2023).

    Author(s)

    • Katrin Hinrichs

      Hinrichs K.

      Professor and Chair
      DVM PhD
      Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania
      Read more about this author

    Copyright Statement

    © All text and images in this publication are copyright protected and cannot be reproduced or copied in any way.
    Related Content

    Readers also viewed these publications

    • Journal Issue

      Veterinary Evidence - Vol 8 N°2, Apr-Jun 2023

      In: Veterinary Evidence
      JUN 07, 2023
    • Proceeding

      NO Laminitis! Virtual Conference - 2021

      By: ECIR - Equine Cushing's and Insulin Resistance Group Inc.
      MAY 02, 2023
    • Proceeding

      BEVA - Annual Congress - Liverpool, 2022

      By: British Equine Veterinary Association
      MAR 20, 2023
    • Journal Issue

      Veterinary Evidence - Vol 8 N°1, Jan-Mar 2023

      In: Veterinary Evidence
      MAR 19, 2023
    • Proceeding

      AVEF - Conférence Annuelle - Reims, 2022

      By: Association des Vétérinaires Équins Français
      MAR 03, 2023
    • Proceeding

      EEHNC - Virtual Congress - 2021

      By: European Equine Health and Nutrition Congress
      FEB 09, 2023
    • Proceeding

      SFT - Theriogenology Annual Conference - Bellevue, 2022

      By: Society for Theriogenology
      JAN 10, 2023
    • Proceeding

      ACVIM & ECEIM - Consensus Statements

      By: American College of Veterinary Internal Medicine
      NOV 11, 2022
    • Journal Issue

      Veterinary Evidence - Vol 7 N°2, Apr-Jun 2022

      In: Veterinary Evidence
      OCT 07, 2022
    • Journal Issue

      Veterinary Evidence - Vol 7 N°3, Jul-Sep 2022

      In: Veterinary Evidence
      OCT 04, 2022
    • Journal Issue

      Veterinary Practice Management Articles - Veterinary Focus

      In: Veterinary Focus
      AUG 05, 2022
    • Chapter

      Nutrition

      In: The Clinical Companion of the Donkey (2nd Edition)
      JUL 09, 2022
    • Chapter

      Pharmacology and Therapeutics

      In: The Clinical Companion of the Donkey (2nd Edition)
      JUL 03, 2022
    • Chapter

      Sedation, Anaesthesia and Analgesia

      In: The Clinical Companion of the Donkey (2nd Edition)
      JUN 05, 2022
    • Chapter

      The Geriatric Donkey

      In: The Clinical Companion of the Donkey (2nd Edition)
      MAY 20, 2022
    • Chapter

      Euthanasia and the Post-Mortem Examination

      In: The Clinical Companion of the Donkey (2nd Edition)
      MAY 20, 2022
    • Chapter

      Appendix 7: Example Diets: for the mature, pregnant and lactating donkey

      In: The Clinical Companion of the Donkey (2nd Edition)
      MAY 12, 2022
    • Chapter

      Appendix 2: Donkey Weight Estimator

      In: The Clinical Companion of the Donkey (2nd Edition)
      MAY 12, 2022
    • Chapter

      Appendix 1: The Clinical Examination

      In: The Clinical Companion of the Donkey (2nd Edition)
      MAY 12, 2022
    • Chapter

      Appendix 5: Monitoring your Donkey’s Quality of Life

      In: The Clinical Companion of the Donkey (2nd Edition)
      MAY 12, 2022
    • Chapter

      Appendix 6: Professional record of Assessment for Quality of Life

      In: The Clinical Companion of the Donkey (2nd Edition)
      MAY 12, 2022
    • Chapter

      Appendix 3: Body Condition Scoring

      In: The Clinical Companion of the Donkey (2nd Edition)
      MAY 12, 2022
    • Chapter

      Appendix 4: Parameters: Biochemistry and Haematology

      In: The Clinical Companion of the Donkey (2nd Edition)
      MAY 12, 2022
    • Chapter

      The Care of the Foal

      In: The Clinical Companion of the Donkey (2nd Edition)
      MAY 12, 2022
    • Chapter

      Approach to the Dull Donkey

      In: The Clinical Companion of the Donkey (2nd Edition)
      MAY 07, 2022
    • Load more
    Provided by:
    Society for Theriogenology

    The Society for Theriogenology is an organization of veterinarians dedicated to animal reproduction, whose mission is to promote standards of excellence in reproductive medicine, to provide outreach and education to veterinarians, and to foster continual improvements in theriogenology.

    Learn more
    Back To Top
    Become a member of IVIS and get access to all our resources
    Create an account
    Sign in
    Leading the way in providing veterinary information
    About IVIS
    • Mission
    • What we do
    • Who we are
    Need help?
    • Contact
    Follow IVIS
    • Twitter
    • Facebook
    International Veterinary Information Service (IVIS) is a not-for-profit organization established to provide information to veterinarians, veterinary students, technicians and animal health professionals worldwide using Internet technology.
    Support IVIS
    © 2023 International Veterinary Information Service
    • Disclaimer
    • Privacy Policy