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Utilizing a Wound Healing Assay to Study Canine Trophoblast Physiology in vitro

Author(s):

J.M. Gullaba and M.A. Kutzler

In: SFT - Theriogenology Annual Conference - Louisville, 2013 by Society for Theriogenology
Updated:
AUG 10, 2013
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Introduction
The wound-healing assay is used to study a cell’s ability to proliferate and migrate in culture. Interleukin-8 (IL8) and tissue inhibitor of metalloproteinase-2 (TIMP2) stimulate and inhibit, respectively, term human trophoblast proliferation and migration in vitro. We hypothesized that term canine trophoblasts (TCTs) would respond similarly. The objectives were to compare wound healing rates in TCTs at increasing concentrations of IL8 and TIMP2. [...]

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The Society for Theriogenology is an organization of veterinarians dedicated to animal reproduction, whose mission is to promote standards of excellence in reproductive medicine, to provide outreach and education to veterinarians, and to foster continual improvements in theriogenology.

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