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Validation of a Fixable Stain for Assessing Viability of Stallion Sperm
E.M. Ligon, C.C. Love, D.D. Varner
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Most sperm viability assays utilize unfixed sperm. Fixable stains have been developed to evaluate the viability of somatic cells but this methodology has not been validated for equine sperm; hence, the objective was to compare a fixable stain with conventional methods. We hypothesized that sperm viability measures are similar when comparing staining techniques for fixed and unfixed equine sperm. Using a protocol adapted in our laboratory, a fixable staining method was compared with commonly used methods for examining sperm viability in unfixed specimens, i.e, 1) SYBR-14 and propidium iodide (SYPI), 2) Pisum sativum agglutinin and PI (PSAPI), 3) NucleoCounter (NC) which utilizes PI, and 4) eosin nigrosin (EN) ...
Equine, sperm, viability, assays, fixation
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Affiliation of the authors at the time of publication
Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas
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