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  4. SFT - Theriogenology Annual Conference - Fort Collins, 2017
  5. Progesterone Evaluation iIn Mares: Comparison Between Endocrine Laboratories
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Progesterone Evaluation iIn Mares: Comparison Between Endocrine Laboratories

Author(s):
Palmer B.D.,
McCue P. and
Ferris R.A.
In: SFT - Theriogenology Annual Conference - Fort Collins, 2017 by Society for Theriogenology
Updated:
AUG 05, 2017
Languages:
  • EN
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    Read

    An accurate assessment of blood progesterone concentrations is critical for optimal reproductive management of broodmares. The goal of this observational study was to compare progesterone concentrations determined by six different endocrine laboratories in a series of identical plasma samples from mares in various stages of reproduction. Blood samples were collected from nine different Quarter Horse mares at various stages of the estrous cycle. Blood was collected into tubes containing EDTA, centrifuged within 10 minutes (1,000 x g for 10 minutes), plasma removed and subdivided into six identical aliquots and frozen at -10°C. Plasma samples were shipped to commercial laboratories on ice using an overnight courier. Four commercial laboratories reported using a radioimmunoassay (RIA) for analysis, while one laboratory used an enzyme immunoassay (EIA) and one laboratory used an enzyme linked fluorescent immunoassay (ELFA). Data are presented as the mean ± SD. Progesterone concentrations for each sample from all six laboratories were within two standard deviations of the mean (i.e. no statistical outliers). However, considerable variation was noted between laboratories for the two samples with the highest P4 concentrations (Sample 1; 8.8±2.8 ng/ml; Sample 3; 6.1±1.9 ng/ml; Figure). [...]

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    How to reference this publication (Harvard system)?

    Palmer, B. D., McCue, P. and Ferris, R. (2017) “Progesterone Evaluation iIn Mares: Comparison Between Endocrine Laboratories”, SFT - Theriogenology Annual Conference - Fort Collins, 2017. Available at: https://www.ivis.org/library/sft/sft-theriogenology-annual-conference-fort-collins-2017/progesterone-evaluation-iin-mares-comparison-between-endocrine-laboratories (Accessed: 01 October 2023).

    Author(s)

    • Palmer B.D.

      DVM
      Department of Biomedical Sciences, College of Veterinary Medecine & Biomedical Sciences, Colorado State University
      Read more about this author
    • Patrick McCue

      McCue P.

      Professor
      BA DVM PhD Dipl ACT
      Equine Reproduction Laboratory, College of Vet Medicine and Biomedical Sciences, Colorado State University
      Read more about this author
    • R. Ferris

      Ferris R.A.

      DVM, MS, Diplomate ACT
      Summit Equine,
      Read more about this author

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    Society for Theriogenology

    The Society for Theriogenology is an organization of veterinarians dedicated to animal reproduction, whose mission is to promote standards of excellence in reproductive medicine, to provide outreach and education to veterinarians, and to foster continual improvements in theriogenology.

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