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Morphological Evaluation of the Stallion Spermatozoa Through Three Staining Methods
E. Jouanisson, Y. Murcia, G...
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Evaluation of sperm morphology is part of the assessment of fertility in human or animal reproduction. The analysis can be performed using different techniques, including the use of staining methods. In our prospective study, morphology of the sperm of the horse was evaluated using three staining methods: Diff Quik® (Siemens Healthcare Diagnostics Inc., Deerfield, IL), Eosin-Nigrosin® (RAL Diagnostics, Martillac, FR) and Spermblue® (Microptic Automatic Diagnostic Systems, Barcelona, SP), a stain used for human sperm. Our hypotheses were that (1) Spermblue® would allow an easier reading of the sperm morphology of the horse and allow a better identification of sperm abnormalities, (2) there would be differences in classification of sperm morphology depending on the evaluator's experience. Semen was obtained from various breeds of horses. [...]
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