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Expression Of Maturation-Associated Genes in Equine Cumulus Cells During in-vitro Maturation is Affected by the Ratio of Oocyte-Cumulus Complexes to Medium
Author(s):
Y. Murcia, H. Guerrero, O. Sanchez...
Updated:
AUG 05, 2017
Languages:
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Little information is available on the factors affecting oocyte quality after in vitro maturation (IVM) in the horse. In vivo, expansion of the cumulus cells (CC) and ovulation is dependent on the expression of amphiregulin (AREG), epiregulin (EREG), prostaglandin endoperoxide synthase 2 (PTGS2) and protein-induced tumor necrosis factor 6 (TNFAIP6). In horses, to date only PTGS2 has been identified in the CC during IVM. We hypothesize that health and function of CC are altered by the density of cumulus-oocyte complexes (COC) during IVM. Our objectives were to measure AREG, EREG, PTGS2 and TNFAIP6 mRNA levels and rates of apoptosis in equine CC after culture of COCs at different densities. [...]
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