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Role of Biofilm in Infectious Endometritis in the Horse
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Treatment of chronic bacterial endometritis with antimicrobials is often unsuccessful. Bacteria have developed multiple mechanisms to tolerate antimicrobial treatment, which include the production of biofilm. Therefore, this project aimed to investigate the localization of bacteria within a biofilm, determine the best diagnostic techniques to detect a biofilm, and evaluate host immune reaction to bacteria in a biofilm using a model of equine bacterial infection. We hypothesized that a bacterial biofilm would be detected in focal areas on the surface of the endometrium, Bouin’s solution would be the ideal fixative for detection of biofilm matrix, and the host immune response would be similar in areas with and without the presence of a biofilm. [...]
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