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Fusion of Boar Sperm With Nanoliposomes Prepared from Synthetic Phospholipids
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Liposomes are artificial membrane vesicles that can be used to test and model the functions and interactions of various biological membranes, as a carrier system to deliver biologically active substances into the cells, or to incorporate lipids into the plasma membrane of target cells to modify membrane structure-function relationships. Sperm plasma membrane undergoes lipid modification during maturation in epididymis, and during capacitation in the female reproductive tract to facilitate fertilization. Natural variation in the amounts and composition of lipids in the sperm plasma membrane may contribute to the species-specific sperm sensitivities to handling and storage conditions. Boar sperm are notoriously susceptible to membrane damage, and are resistant to compositional alteration by artificial liposomes.
Nanoliposomes, boar sperm, flow cytometry, fusion efficiency
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Affiliation of the authors at the time of publication
Department of Animal and Poultry Science, University of Guelph, Guelph, ON, Canada
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