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Effect of Different Concentrations of Glycerol in Cryopreservation of Gaddi Goat Semen
A. Sharma, P. Sood, J.K. Chaudhary
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Abstract
Goats have greater importance under small familiar systems in India. The unabated decline in Gaddi males of superior genetic merit due to extensive culling and unplanned breeding (inbreeding) accentuates the importance of conserving superior males and their use for artificial insemination. The available literature is unequivocal about the importance of glycerol during sperm cryopreservation. However, the optimal level seems to vary between species and breeds. The present study aimed to evaluate the effect of different concentrations of glycerol (6, 7, and 8%) on the quality of thawed semen from Gaddi bucks. A total of 180 ejaculates from six Gaddi buck were frozen in Tris citrate extender containing 10% of Egg Yolk with 6, 7, or 8% of glycerol. Sperm quality parameters in thawed semen (morphological abnormalities, sperm viability, progressive motility, and HOST response) were compared; in addition, the percentage of change in thawed semen with respect to fresh was determined. No effect of concentration of glycerol was observed on progressive motility and HOST response. Sperm viability was better in semen frozen at 6 and 8% (45.26±1.32% and 45.10±2.81% respectively) in comparison with 7% of glycerol (34.81±2.78%, P<0.05). Lower sperm morphological abnormalities were observed in semen frozen at 6% of glycerol (7.93±0.28, P<0.05) in comparison with 7% (9.18±0.69%) and 8% (9.90±0.55%). A fertility rate of 41.25% was achieved following AI with semen frozen containing 6% of glycerol. In conclusions, 6% of glycerol was a valid option to cryopreservation of semen from Gaddi buck, resulting in better viability and lower abnormalities. In addition, a good fertility response was observed.
Resumen
Las cabras están teniendo mayor importancia en los pequeños sistemas familiares en India. Sin embargo, la disminución deliberada de machos Gaddi de alto merito genético debido a la eliminación excesiva y a los cruzamientos no planificados (consanguinidad) acentúan la importancia de conservar machos superiores y su uso para inseminación artificial. La literatura disponible ha confirmado la importancia del glicerol durante la criopreservación espermática. Sin embargo, el nivel óptimo parece variar entre especies y razas. El propósito de este estudio fue evaluar el efecto de diferentes concentraciones de glicerol (6, 7 y 8%) sobre la calidad del semen descongelado de machos de raza Gaddi. Un total de 180 eyaculados de seis machos fueron congelado en diluyente a base de Tris citrato conteniendo 10% de yema de huevo con 6, 7 u 8% de glicerol. Los parámetros de calidad espermática en el semen descongelado (motilidad progresiva, viabilidad, anomalías morfológicas y respuesta a HOST) fueron comparadas, además el porcentaje de cambio de cada parámetro con respecto al semen fresco fue determinado. No se observó un efecto del nivel de glicerol sobre la motilidad progresiva y la respuesta al HOST. La viabilidad espermática fue mejor en el semen congelado con 6 y8% de glicerol (45,26±1,32% y 45,10±2,81% respectivamente) en comparación con el semen congelado con 7% de glicerol (34,81±2,78%, P<0,05). Menos morfoanomalías fueron observadas en el semen congelado con 6% de glicerol (7,93±0,28, P<0,05) en comparación con el congelado con 7% (9,18±0,69%) y 8% (9,90±0,55%). Una tasa de 41,25% de fertilidad fue alcanzada luego de la inseminación con semen congelado con &% de glicerol. En conclusión, 6% de glicerol es una opción validad para la criopreservación de semen de machos cabríos Gaddi, resultando en una mayor viabilidad y menos morfoanomalías espermáticas. Además, una buena fertilidad fue observada.
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About
Affiliation of the authors at the time of publication
CSK Himachal Pradesh Krishi Vishvavidyalya, Palampur-Himachal Pradesh 176062, India (Sharma, Sood); Central Agricultural University, Aizwal, Mizorum, India (Chaudhary)
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