Diagnostic Application of Serum Adenosine Deaminase Level in Canine Infectious Disease

Adenosine deaminazse is a cytoplasmic enzyme, which is essential for the proliferation and differentiation of lymphocytes. increased aDa is derived mainly from increased immune cells numbers, whereas inhibition or genetic deficiency of aDa resulted in immunodeficiency.

In this study, three canine chronic infectious diseases (visceral leishmaniosis, ehrlichiosis and brucellosis) were selected for evaluation of serum aDa alteration in compare with clinically healthy dogs.

Different diagnostic methods were used for detection ehrlichiosis, brucellosis and leish- maniosis among 378 serum sample that obtained from sick owned dogs. on the other hand, 50 healthy dogs which, referred for health checks up and vaccination and their hematological and biochemical evaluation were in normal limit were randomly selected as control group. total aDa level, were measured by a commercial kit via autoanalyzer in all selected samples.

Total aDa level were measured as 7.5±.0.8 iu/l, 5.7±0.48 iu/l, 5.2±0.63 iu/l and 2.26 ±0.25 iu/l in visceral leishmaniosis, ehrlichiosis, brucellosis and healthy dogs respec- tively. there was no significant difference in aDa level between diseased groups but aDa level in leishmaniosis, ehrlichiosis and brucellosis groups showed a significant increase in compare with healthy animals.

Based to the results of the present study, total serum aDa level may be used as an important biomarker for the diagnosis of canine chronic infection disease and can be added to the other routine biochemical tests that are used in the veterinary clinical pathology.