Debarking by Botox Injection

The present research attempts to provide a reliable solution for barking dogs and keeping them in urban environment, using a non-surgery, pharmacological, reversible method. We also conduct a review to find the precise appropriate location for medicine injection and the exact, needed amount of the medicine to be injected as well as diagnosis of its possible side- effects after injection.

For this purpose, we selected 6 mature male dogs seemingly 1 to 2 years of age from big race, and then we divided them into three groups of two members including two experimental groups (A, B) and a control group of C. Then the dogs were kept in normal situation in cages, until they were prepared to be injected medicine. Afterwards, they were undergone anesthesia process under sterile situation in public surgery room. We pulled the tongue of the animal to one side using light and biopsy forceps in order to gain access to glottis and sound cords. Using Needle no. 28 and insulin syringe, we injected two different doses from clostridium botulinum type A, totally 150 units (to each dog of group A) and 100 units ( to each dog of group B) in the place of muscles that lead to sound cords, in a two-way method. Also, we injected the same amount of physiology serum to each dog of the control group (C) in the same place with the same method. (2) After injection, the dogs were kept for 3 months and their sound changes were recorded each day under normal conditions.

The results of this study indicated that during the first month after injection, the experimental groups refused to bark when recording the sound. They had become calm; however, these changes were not significant. Since such a study had not already carried out in veterinary, the results that have been obtained through this study can be regarded as a base for pilot future researches on this issue. For this purpose, we need clostridium botulinum type A in various doses in different parts of glottis.