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Differentiation of Six Pakistani Camel Breeds by Molecular Genetics Analysis
A.S. Qureshi, M.G. Shah, M...
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Introduction
Camel is a queer animal; an inhabitant of hot deserts and also cold mountains. Camel population in Asia is not genetically homogeneous rather it is a multitude of breeds adapted to diverse cultural, ecological and economic contexts. Genetics could be a powerful tool to modify animal growth rate and body composition, and has been largely used to adapt animal production to the market requirements (Guernec et al., 2003). In the present project an effort has been made to determine genetic analysis for genetic differences in the local camel breeds, which have so far been differentiated only on the basis of phenotypic appearance, geographical location or on the tribal ownership.
Materials and Methods
A total of 157 camels belonging to six different Pakistani breeds Viz. Marecha (n= 26), Dhatti (n= 24), Larri (n= 27), Kohi (n= 30), Campbelpuri (n= 25) and Sakrai (n= 25) were included in this study. These animals belonged to different ecological zones of Pakistan and presented a high variation in their coat colour. Genomic DNA was isolated from their hairs using NucleoSpin Tissue kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol. For primer design sequences from the NCBI GenBank were used. PCR reactions were carried out using UNO thermo cycler (Biometra, Germany) in a total volume of 25 µl containing 2.5 mM, MgCl2, 0.2 mM dNTP 1U Taq DNA Polymerase (Genaxxon, Germany) 0.2 µM of forward and reverse primer and 100 ng genomic DNA. After an initial denaturation with 94°C for 2 min 35 cycles were done each consisting with 94 °C for 1 min, 56 °C (primer pairs MN-1B up and MN-1B low) for 30 sec and 72 °C for 40 sec. The final step lasted for 10 min at 72 °C. PCR amplified fragments were excide from 2% agarose gel and purified using Gene Clean II Kit […]
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