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Oocytes and ICSI: Where Are We Now?
N. Lewis
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Introduction
The efficiency of assisted reproduction techniques in the horse have improved greatly in the last decade. Several techniques are now used clinically as a treatment for infertility, and post mortemas a form of genetic salvage. Oocyte transfer (OT), gamete intra- fallopian transfer (GIFT) and intra-cytoplasmic sperm injection (ICSI), bypass most of the female tubular reproductive tract and can therefore be used to circumvent associated pathologies and establish surrogate pregnancies in recipient mares. Relevant pathologies include:
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Ovulation failure.
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Blocked oviducts.
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Severe endometritis.
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Cervical incompetency. In addition, GIFT and ICSI protocols require very low numbers of spermatozoa providing a valuable tool in cases of stallion subfertility.
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