British Equine Veterinary Association
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Equine Influenza: Can Science Facilitate Safer International Movement of Horses?

Author(s):

Paillot R., Prowse L., Montesso F...

In: BEVA - Annual Congress - Manchester, 2013 by British Equine Veterinary Association
Updated:
SEP 14, 2013
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Aims: The aims of this study were firstly, to compare the results of different diagnostic tests for the detection of equine influenza (EI) after infection of vaccinated horses and secondly, to determine if nasopharyngeal swabs were more sensitive than nasal swabs for detection of EI virus (EIV). 

Methods: Two EIV naïve ponies and 5 vaccinated ponies were experimentally infected with A/eq2/Richmond/1/07. Nasal and nasopharyngeal swabs were taken daily from all infected ponies for 14 days after challenge infection. The 5 vaccinates were introduced to 3 groups of 2 naïve sentinel ponies for 48 h on Day 2 (transmission 1), Day 4 (transmission 2) and Day 6 (transmission 3) after challenge. Presence of EIV in clinical samples was determined by EIV antigen capture ELISA and EIV RT-qPCR. Positive samples were inoculated into embryonated eggs. [...]

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