AAEP Annual Convention San Diego 2008
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Evaluation of Immune Responses in Foals Post- Vaccination With a West Nile Virus Vaccine (Live Flavivirus Chimera)

Author(s):
Gutierrez C.,
Brown II S.E.,
Long M.T. and
Horohov D.W.
In: AAEP Annual Convention - San Diego, 2008 by American Association of Equine Practitioners
Updated:
DEC 10, 2008
Languages:
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1. Introduction

In this study, we investigated the foal’s immunesystem response to a chimeric West Nile Virus (WNV) vaccine to better understand the immunity that is conferred post-vaccination. Additionally, we studied how maternal antibody interference affects vaccination with a chimeric WNV vaccine [a]. This provides valuable information to practitioners in terms of when to administer such preparations in an effort to maximize the immune response in foals.

2. Materials and Methods

Serum titers, cell-mediated cytokine parameters, and in vitro stimulation of peripheral blood mononuclear cells (PBMC) were performed on 40 3- and 5-mo-old foals post-vaccination with a WNV chimeric vaccine. Age-dependent controls were also sampled.

3. Results

A significantly (p < 0.05, repeated measures-analysis of variance [RM-ANOVA]) increased granzyme B expression was detected in the 5-mo-old vaccinated foals. WNV-stimulated PBMC from the 5-mo-old foals exhibited a significantly increased expression of IL-2, perforin, and TGF-β compared with non-vaccinated controls; additionally, a trend with IFN-γwas observed. A significant titer response post-vaccination was not detected.

4. Discussion

This study shows that vaccinating foals with a single dose of a chimeric WNV vaccine in the face of maternal antibodies does produce a cell-mediated response that is indicative of a Th-1 mediated response with stimulation of cytotoxic T cell activity.

This study was funded and performed by Intervet/ Schering-Plough Animal Health in collaboration with the Maxwell H. Gluck Equine Research Center, the University of Florida’s College of Veterinary Medicine, and Hagyard Equine Medical Institute. The authors wish to thank Sarah Beachboard, DeAnne Fanta, and David Gosche of EDART Laboratory as well as Marc McLean and Crestwood Farm.

Footnotes

  1. PrevenileTM, Intervet/Schering Plough Animal Health, Millsboro, DE 19966.
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How to reference this publication (Harvard system)?

Gutierrez, C. et al. (2008) “Evaluation of Immune Responses in Foals Post- Vaccination With a West Nile Virus Vaccine (Live Flavivirus Chimera)”, AAEP Annual Convention - San Diego, 2008. Available at: https://www.ivis.org/library/aaep/aaep-annual-convention-san-diego-2008/evaluation-of-immune-responses-foals-post-vaccination-a-west-nile-virus-vaccine-live-flavivirus (Accessed: 04 October 2023).

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