AAEP Annual Convention San Antonio 2011
Back to Table of Contents
Add to My Library
Comments
Share:

Equine Bone Marrow-Derived Stem Cells: Comparing the Ilium and the Sternum

Author(s):

M.K. Adams, L.G. Goodrich, S. Rao...

In: AAEP Annual Convention - San Antonio, 2011 by American Association of Equine Practitioners
Updated:
NOV 22, 2011
Languages:
Back to Table of Contents
Add to My Library

Both the sternum and ilium offer a rich supply of stem cells that have similar growth rates. The highest concentration of stem cells is in the first 5 ml bone marrow aspirate. Authors’ addresses: Clinical Sciences (Adams, Rao, and Olea-Popelka) and Equine Orthopaedic Research Center (Goodrich, Phillips, Kisiday, and McIlwraith), 300 West Drake Road, Colorado State University College of Veterinary Medicine and Biomedical Sciences, Fort Collins, CO 80521; e-mail: [email protected].

1. Introduction

The two sites of equine bone marrow derived mesenchymal stem cells (BMDMSC) harvest are the sternum and ilium, and site selection is based primarily on practitioner preference. The goal of this study was to determine the effects of harvest site and harvest fraction on stem cell quantity and rate of growth. We hypothesized that cell concentration would be higher in the sternum than the ilium and higher in the first fraction of marrow compared with subsequent fractions. Furthermore, we hypothesized that cell growth rates would not differ between sites.

2. Materials and Methods

Two sequential 5-ml marrow samples were taken from the sternum and ilium of seven horses before euthanasia. Nucleated cell counts (NCCs) were obtained for all samples pre- and post-marrow processing. Cells were expanded in culture for three passages, and NCCs were obtained at each passage.

3. Results

There was no significant difference (p > 0.05) between the NCCs of the first sternum aspirate and the first ilium aspirate. However, the NCCs of the first 5-ml aspirate were significantly higher than the second 5-ml aspirate for both sites (p < 0.05). There was no significant difference between growth rates for any of the groups (p > 0.05).

4. Discussion

These data should give practitioners confidence that both the sternum and ilium offer a rich supply of stem cells that have similar growth rate characteristics.

This study was funded in part by a PVM Student Grant from the Center for Companion Animal Studies at the Colorado State University.

View full text
Back to Table of Contents
Add to My Library

Ask the author

0 comments
Submit

Affiliation of the authors at the time of publication

Clinical Sciences (Adams, Rao, and Olea-Popelka) and Equine Orthopaedic Research Center (Goodrich, Phillips, Kisiday, and McIlwraith), 300 West Drake Road, Colorado State University College of Veterinary Medicine and Biomedical Sciences, Fort Collins, CO 80521, USA

Copyright Statement

© All text and images in this publication are copyright protected and cannot be reproduced or copied in any way.

Readers also viewed these publications

Provided by:

The AAEP represents nearly 9,300 veterinarians and veterinary students in 61 countries who cover a broad range of equine disciplines, breeds and associations. The AAEP is primary resource for education, professional development and ethical standards for its members. The AAEP and its members are recognized as the voice and authority for the health and welfare of the horse. The AAEP conducts regular strategic planning every three to four years in order to establish priorities and set direction for the association over the current planning horizon.  The AAEP is a respected source of information for influencing public policy.  

Learn more
Back To Top