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Influence of Technical Settings on CASA Motility Parameters of Frozen Thawed Stallion Semen
M.K. Hoogewijs, J.L. Govaere, T...
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1. Introduction
The widespread use of frozen thawed semen in the equine breeding industry creates opportunities for breeders and stallion owners. Semen can be shipped all over the world so that any stallion may become available for any given mare. Inseminations with frozen thawed semen, however, are less successful compared with inseminations with cooled and, especially, fresh semen. To optimize pregnancy outcome, frozen thawed semen should fulfill some criteria such as a minimal total number of spermatozoa per dose and a minimal progressive motility, e.g., the insemination dose for conventional artificial insemination (AI) using frozen thawed semen should at least contain 240 x 106 progressively motile spermatozoa (PMS).1 The progressive motility (PM) should at least be 30%,1 whereas the World Breeding Federation for Sport Horses advocates a PM of 35%.2
Computer-assisted sperm analysis (CASA) is accepted as an accurate tool for motility analysis. A number of motility settings for CASA have been described. In this paper, we studied the repeatability of CASA. Furthermore, the influence of CASA motility settings on several sperm parameters when analyzing frozen thawed semen with two different settings was studied. [...]
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About
Affiliation of the authors at the time of publication
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133 B-9820, Belgium
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