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Effect of Hyaluronic Acid and Betamethasone on Interleukin-1 - Treated Chondrocyte Pellets
E. Schaefer, A.A. Stewart, M.C...
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1. Introduction
A previously published study performed on equine chondrocyte pellet cultures treated with interleukin-1 (IL-1) showed that the addition of a high molecular weight hyaluronic acid (HA) combined with triamcinolone had protective effects on IL-1–induced proteoglycan (PG) matrix catabolism. Our hypothesis was that administration of HAa at a molecular weight of 3 million Daltons, alone or in combination with betamethasone,b could equally mitigate effects of IL-1– induced chondrocyte PG matrix catabolism.
2. Materials and Methods
Normal chondrocyte pellets were isolated from seven horses. Chondrocyte pellet cultures from each horse were treated with fresh control media, IL-1 media, or IL-1 media containing HA, betamethasone, or betamethasone and HA. New PG synthesis and release was determined by 35SO4 incorporation into the pellet or release into the media, respectively. Total glycosaminoglycan (GAG) content in the pellets and media was determined using a dimethylmethylene blue binding assay. Statistical significance was determined using repeated measures analysis of variance on statistical softwarec with a p value of <0.05 considered to be significant. [...]
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About
Affiliation of the authors at the time of publication
University of Illinois, College of Veterinary Medicine, Large Animal Clinic, 1008 West Hazelwood Drive, Urbana, Illinois 61802, USA
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