AAEP Annual Convention Anaheim 2012
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Use of Mesenchymal Stem Cells or Autologous Conditioned Serum to Modulate the Post-Mating Inflammatory Response in Mares

Author(s):
Ferris R.A.,
Frisbie D.D. and
McCue P.
In: AAEP Annual Convention - Anaheim, 2012 by American Association of Equine Practitioners
Updated:
DEC 05, 2012
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Autologous conditioned serum (ACS) and bone-derived, culture-expanded mesenchymal stem cells (MSCs) were able to modulate the inflammatory response to spermatozoa in mares.

1. Introduction

Current treatments for post mating induced endometritis (PMIE) focus on aiding the uterus in removal of inflammatory products, but these treatments do not modulate the inciting inflammatory response. Systemic glucocorticoid administration has potential side effects such as laminitis, ovulation failure, suppression of the immune system, and so forth. Biological treatments for immunomodulation have been used in human and veterinary medicine for more than 10 years.

2. Materials and Methods

Two experiments were performed with six mares in each study to evaluate the effects of intrauterine administration of ACS,a  dexamethasone, or a placebo (experiment 1) or allogeneic MSCsb  or placebo administration (experiment 2) on the inflammatory response to spermatozoa, using clinical and biochemical end points.

3. Results

Treatment with ACS and MSCs significantly (P < 0.05) reduced the number of neutrophils in the uterine lumen 6 hours after challenge with spermatozoa. An increase (P < 0.05) in the anti-inflammatory cytokine interleukin (IL)-1Ra was observed after treatment with MSCs. There was no difference in IL-1Ra concentration in mares treated with ACS, dexamethasone, or a placebo. 

4. Discussion

MSCs or ACS was able to modulate the immune response to spermatozoa in mares. The effect may be due to an increase in IL-1Ra in MSC-treated mares, but other bioactive molecules may be responsible for the decrease in neutrophils in ACS-treated mares. Future studies evaluating the effect of MSCs and ACS on pregnancy rates should be performed.

Footnotes

a IRAP II, Arthrex Vet Systems, Bonita Springs, FL 34108.
b Allogeneic mesenchymal stem cells, Advanced Regenerative Therapies, Fort Collins, CO 80523.
c Dexamethasone, 2 mg/mL, VetOne, Boise, ID 83705.

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How to reference this publication (Harvard system)?

Ferris, R., Frisbie, D. D. and McCue, P. (2012) “Use of Mesenchymal Stem Cells or Autologous Conditioned Serum to Modulate the Post-Mating Inflammatory Response in Mares”, AAEP Annual Convention - Anaheim, 2012. Available at: https://www.ivis.org/library/aaep/aaep-annual-convention-anaheim-2012/use-of-mesenchymal-stem-cells-or-autologous-conditioned-serum-to-modulate-post-mating-inflammatory (Accessed: 01 October 2023).

Author(s)

  • R. Ferris

    Ferris R.A.

    DVM, MS, Diplomate ACT
    Summit Equine,
    Read more about this author
  • D.D. Frisbie

    Frisbie D.D.

    MS, DVM, PhD, Dipl. ACVS, Dipl. ACSMR
    Clinical Sciences Department, College of Veterinary Medicine and Biomedical Sciences, Colorado State University
    Read more about this author
  • Patrick McCue

    McCue P.

    Professor
    BA DVM PhD Dipl ACT
    Equine Reproduction Laboratory, College of Vet Medicine and Biomedical Sciences, Colorado State University
    Read more about this author

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