ISEET - International Symposium on Equine Embryo Transfer and Technology

ISEET 2016

Program

Scientific Program (Download)

Thursday, June 30 - Travel ICAR-Tours to Gent

The ICAR meeting in Tours ends around 13:00. Delegates can take the TGV train from Tours to Brussels and change in Brussels to a regular train to Gent. The TGV station in Tours is literally across the street from the conference center. At present, there are trains departing at 2-hr intervals. The journey Tours to Brussels takes 4hrs. You need 1hr to go from Brussels by train to Gent (center of the city). On Friday July 1st, the same train schedules apply. Alternatively, we can arrange for a bus from Tours to Gent on June 30th departing at 14:00. The bus ride takes 5-6hrs depending on traffic. Delegates that travel by bus would arrive on Thursday around 20:00 at their hotel in the city center. Delegates will be asked if they would like to take the bus and if more than 30 people sign up, we will organize a bus.

Friday, July 1 - Welcome

During the day: Delegates can spend the day in Gent discovering the city. Information about guided tours, opening hours for museums and list of good restaurants in the city center will be made available to all delegates.

In the evening: At 17:00 on Friday, registration and welcome reception will take place at the conference center (Sint Augustijnenklooster). The conference center is in the center of the city at 10 min walking distance from the main touristic places and hotels (Google Map).

17:00-18:15	Registration / Drinks / Commercial Booths Visit / Posters Viewing / Visit of the Old Library of the Sint Augustijnen Abby
18:15-19:00	Welcome address and Keynote Lecture The Horse Catalyzed Birth of Modern Veterinary Medicine in 18th Century France - Peter Bols (view)
19:00-22:00	Gala Dinner / Visit of the Old Library

Saturday, July 2 - Day 1

Session 1 - Jean-François Bruyas (Moderator)
8:30-9:00	Keynote Lecture - Cryopreservation of Equine Embryos – Ed Squires (view)
9:00-9:15	Easy, quick and cheap technique to cryopreserve Welsh B pony blastocyst - F. Guignot – Abstract #6
9:15-9:30	Vitrification of large equine embryos following manual or micromanipulator-assisted blastocoele collapse - R. Ferris – Abstract #31
9:30-9:45	In vitro evaluation by DAPI staining of fresh, cooled and vitrified donkey embryos - D. Panzani – Abstract #45
9:45-10:00	Toxicity of a vitrification protocol for equine immature oocytes - H. DeSiqueira Canesin – Abstract #63
10:00-10:45	Coffee break
Session 2 - Tom Stout (Moderator)
10:45-11:00	Preliminary morphokinetic annotation data for early equine embryo development in vitro using a time-lapse imaging system - K. Schnauffer – Abstract #47
11:00-11:15	Combining Time-Lapse Imaging and Next Generation RNA-Sequencing to Assess Equine Embryo Developmental Potential - S. Chavez – Abstract #65
11:15-11:45	Keynote Lecture - Clinical Applications of Preimplantation Genetic Testing in Equine, Bovine and Human Embryos – Carolina Herrera (view)
11:45-12:00	Application of ultrasound technique in early fetal gender determination – the field study - S. Martinez Zuviria – Abstract #15
12:00-12:15	Influences of conceptus sex on its development during early equine pregnancy - C. Aurich – Abstract #10
12:15-13:45	Lunch
Session 3 - Claudia Klein (Moderator)
13:45-14:00	Results and limitations on the use of sex-sorted semen in conventional ET programs in Polo Argentino breed - A. Lascombes – Abstract #67
14:00-14:15	Overnight Shipping of equine oocytes from remote locations to an ART laboratory enables access to the flexibility of ovum pick up ICSI and embryo cryopreservation technologies - C. Galli – Abstract #68
14:15-14:30	Effects of different media systems and osmolarity on equine blastocyst development after ICSI - Young Ho Choi – Abstract #22
14:30-14:45	Successful in vitro production of mammalian embryos: a strict quality management approach - C. Herrera – Abstract #52
14:45-15:00	Production of equine embryos in vitro using conventional intracytoplasmic sperm injection and a complete human embryo culture system - C.A. Leisinger – Abstract #60
15:00-15:45	Coffee break
Session 4 - Katrin Hinrichs (Moderator)
15:45-16:15	Keynote Lecture - In vitro Production of Equine Embryos and Cloning: Today’s Status – Marc Maserati and Adrian Mutto (view)
16:15-16:30	Rescuing equine cloned embryos by aggregation - A. Gambini – Abstract #27
16:30-16:45	Enrichment of somatic cells from frozen-thawed equine semen using fluorescence-activated cell sorting - J. G. Brom-de-Luna – Abstract #56
16:45-17:00	Variability in efficiency in a commercial oocyte transfer program - F. Riera – Abstract #9
17:00-17:15	Pregnancy rate and pregnancy loss after transfer of in vivo or in vitro derived equine embryos - P. McCue – Abstract #42
17:15-19:45	Dinner on your own in the city - 5-10 min walk to Graslei with many restaurants
19:45-22:00	Workshop - two parallel workshops on ET and IVP
19:45-22:00	Workshop 1 - Clinical aspects of embryo transfer - McCue, Riera, Aurich Workshop 2 - In vitro production of equine embryos - Hererra, Maserati

Sunday, July 3 - Day 2

Session 5 - Luis Losinno (Moderator)
8:30-9:00	Keynote Lecture - Maternal Recognition of Pregnancy in the Context of Equine Embryo Transfer – Claudia Klein (view)
9:00-9:15	Effects of suppression of estrogen synthesis during early pregnancy in mares - B.A. Ball – Abstract #11
9:15-9:30	Embryo quality grade and recipient ovulation rate affect pregnancy rates in embryo transfer recipient mares - H.G. Pedersen – Abstract #24
9:30-9:45	Effects of asynchronous embryo transfer on the endometrial transcriptome in mares - C. Gibson – Abstract #43
9:45-10:00	Association between equine sperm parameters and ICSI outcome – R. Gonzalez-Castro – Abstract #25
10:00-10:45	Coffee break
Session 6 - Young Ho Choi (Moderator)
10:45-11:00	Glucose uptake and lactate production of equine cumulus-oocyte complexes (COC’s) during in vitro maturation (IVM) – J. Len – Abstract #19
11:00-11:15	Effect of pyruvate addition and oxygen concentration on carbohydrate metabolism during in vitro maturation of equine oocytes – N. Lewis – Abstract #30.
11:15-11:30	Effects of verbascoside treatment during oocyte in vitro maturation on blastocyst development and bioenergetic/oxidative status after ICSI in the horse – N.A. Martino – Abstract #34
11:30-11:45	Developmental Competence of Equine Oocytes is Associated with Alterations in their Cumulus Proteome – J. Walter – Abstract #59
11:45-12:30	Keynote Lecture - Genome Editing in Large Animals – James West (view)
12:15-13:45	Lunch
Session 7 - Cezare Galli (Moderator)
13:45-14:00	Maturation Condition alters the Cumulus Proteome of Equine Oocytes – F. Huwiler – Abstract #62
14:00-14:15	The effect of a simulated physiological oocyte maturation system on the in vitro production of equine embryos – E.S. Metcalf – Abstract #32
14:15-14:30	Dynamics of in vitro maturation of equine oocytes recovered by transvaginal follicular aspiration – M.B. Rodríguez – Abstract #8
14:30-14:45	In Vitro Production of Equine Embryos by Ovum Pick Up and Intracytoplasmic Sperm Injection in Young Mares – R. Sanchez – Abstract #58
14:45-15:00	Factors influencing oocyte recovery and in-vitro production of equine embryos in a commercial OPU/ICSI program – A. Claes – Abstract #39
15:00-15:30	Coffee break
Session 8 - Peter Daels (Moderator)
15:30-16:15	Keynote Lecture - Gametes, Embryos and their Epigenome: Considerations for Equine Embryo Technologies – Pascal Chavatte (view)
16:15-16:30	Effect of the interval from Prostaglandin F2a treatment to ovulation on Embryo Recovery Rate and subsequent Pregnancy Rate in a commercial Equine Embryo Transfer program - J. Cuervo Arango – Abstract #17
16:30-16:45	Factors Affecting Embryo Recovery: Retrospective Study based on Commercial Embryo Transfer – E. Squires – Abstract #23
16:45-17:00	Doppler ultrasound for assessment of follicular response to induction of ovulation in mares – M. Alvarenga – Abstract #33
17:00-17:15	Dominant follicle ablation as a way of preparing embryo recipient mares – F. Saules Ignácio – Abstract #50
17:15-17:30	Closing remarks and planning for the future
17:30-22:00	Buffet Dinner
22:00	End of the ISEET-2016 Conference

ISEET-2016 Conference Posters

#1 - Intrauterine administration of coconut oil to early pregnant mares increases embryonic vesicle size - Sandra Wilsher and WR Allen #2 - Effect of Mesenchymal Stem Cells on Equine Ovarian Follicular Development - Sicilia T Grady, Ashlee E Watts, and Katrin Hinrichs #3 - Is spontaneously double ovulation associate with incidence of EPL after nonsurgical embryo transfer in mares? - Hamed Farnia, N. Largel, S.M. Zuviria, J.I. Lopez Echenique #4 - The Effect of Eight Antioxidants on Cryopreserved Equine Sperm Quality - Zhuangyuan Wu, Jingbo Chen, Yongming Luo, Xinbao Zheng, Hong Dong, Fei Huo #5 - Comparison between open and closed vitrification system in equine collapsed embryos - Weiss, J. Blanco, M. Sanchez, R. Arbouin, C. Schockemöhle, P. #7 - Expression of mesenchymal stem/stromal cell markers and steroid hormone receptors in the equine endometrium - Rink, E., Kuhl, J., Aurich, C., French, H., Nino-Fong, R., Watson, E., Donadeu, F.X. #12 - Cooling equine embryo for 24h prior to vitrification: What are the consequences on its viability? - Maud Caillaud, Estelle Provost And Florence Guignot #13 - Efficiency of two prostaglandin F2a analogs for synchronization of estrus and ovulation in mares - Juliane Kuhl, Jörg E. Aurich, Christine Aurich #14 - Comparison of the efficacy of different single doses of buserelin with hCG for timed ovulation induction in the mare. - Newcombe, JR and Cuervo-Arango, J #16 - Embryo Transfer and its influence on the Polo Argentino breed in the last 25 years Martinez - Zuviria S. and Zagrajczuk A. #18 - The influences of age, laminitis and serum ACTH concentration on embryo recovery from Arabian mares - Jenny Katrine Boye, Valentina Barone and JG Oriol #20 - Thawed stallion sperm refrozen without cryoprotectants activates cow oocytes after intracytoplasmic sperm injection (ICSI) - Jose Len, Dave Kleeman, Jen Kelly #21 - Application of flunixin meglumine at the time of embryo transfer and the effect on the fertility - Okada, C.T.C., Teixeira Martini Segabinazi, L.G., Alvarenga, M.A. #26 - Assessment of fetal sex determination in early equine pregnancy by isolation of fetal cells from the maternal circulation - Martin Köhne, Michaela Prchal-Murphy, Martin Hofer, Ralf Steinborn, Christine Aurich #28 - Attempts to improve synchronization of ovulation and induce superovulation in the mare - Shuaishuai Wu, Nan Li, Shumin Wang, Guocai Han, and Shenming Zeng #29 - Ultrasonographic evaluation of fetal growth between full siblings Arabian foals gestating in their genetic dams and embryo transfer recipients. Merridale-Punter M, Galinelli NC and Oriol JG #35 - Proteomic analysis of the cervical mucus plug during pregnancy in mares - Loux, S.C., Scoggin, K., Troedsson, M. H., Squires, E.L., Ball, B.A. #36 - Report of frozen equine embryos transfer in the Czech Republic - Josef Muller, Jindrich Muller #37 - Influence of mare parity on weight, height, thoracic circumference and vitality of neonatal foals - FJ Affonso, MG Meirelles, MA Alonso, CF Guimaraes, KM Lemes, M Nichi, CB Fernandes #38 - Effect of maternal age on estrogen production in mid to late pregnancy in the mare - MA Alonso, FJ Affonso, MG Meirelles, R Simas, DF Souza, VV Hernandes, M Nichi, MN Eberlin, CB Fernandes #40 - Potential to reduce negative effects of exercise stress on number of pregnancies in a commercial embryo transfer program. - Pinto, M., Miragaya, M., Burns, P., Douglas, R. and Neild, D. #41 - Can pregnancy survival after embryo transfer be predicted based on early ultrasound examinations in the recipient mare? - Morrissey JK, Ferris RA, Trundell D, Loncar K, Burden C, Scofield D, McCue PM #44 - Influence of insemination on regulatory T cells in the peripheral blood of estrous mares - Constanze Hartmann, Wilhelm Gerner, Armin Saalmüller, Christine Aurich #46 - Comparative efficacy of hCG or GnRH analogue (lecirelin acetate) on follicular dynamics, degree of endometrial edema, sexual behavior, ovulation and pregnancy rate in crossbred broodmares - Fayzan Sultan Awan, Muhammad Usman Mehmood, Abdul Sattar, Nasim Ahmad #48 - Can we predict fertility in prepubertal female horses using Anti-Müllerian hormone? - Dragos Scarlet, Natascha Ille, Manuela Wulf, Ingrid Walter, Christine Aurich #49 - The effect of exercise of the donor mare in an embryo transfer program - M. Christoffersen, M. Dige Soegaard, H. Gervi Pedersen and R. Munk Andersen #51 - Effect of firocoxib on ovulation and fertility rates of embryo donor mares - Friso A M, Cyrino M A, Teoro M T, Alvarenga M A #53 - In vitro growth of small equine embryos after vitrification - Celina M Checura and Alexis Powers #54 - Preimplantation Gender Determination on Equine Embryos using LAMP - P. Dini, M. Van Poucke, C. Herrera, L. Peelman and Peter Daels #55 - Comparison of pregnancy rates between acyclic mules and cyclic mares as recipients for embryo transfer - C.E. Camargo, R.C. Macan, S.F. Rechsteiner, E.L. Gastal #57 - Gene expression prior and after vitrification of equine CCO’s - Polenz, M.F., Pereira, G.R., Augusto, C.G., Fiala Rechsteiner, S. #61 - Holding Equine Oocytes in Syngro® Embryo Holding Medium at 4°C. - P. Dini, O. Bogado, M. Hostens and P. Daels #64 - Improving frozen equine sperm motility by the addition of different concentrations of Coenzyme Q10 to a freezing extender - Lagares, MA, Luz, SB, Coutinho, IM, Morais, BM and Stahlberg, R #66 - Transport of Equine Oocytes in Syngro® Embryo Holding Medium using a Passive Cooling System (Equitainer®) - Kaatje Danielle Ducheyne, Francisco Guaimas, Romina Santa Cruz, Julieta Mertian, Nicolás Matiás Ortega, Peter Daels and Adrián Mutto #69 - Efficacy of a single injection of buserelin acetate in saline solution and a single injection of hCG for the induction of ovulation in mares - L. Normandin, C. Frilley, A. Cochard, H Terris, J.-F. Bruyas #70 - Is there a repeatability of the size of follicles at the ovulation within individuals in mares - C. Lefrançois, J.-F. Bruyas #71 - Effect of asynchronous embryo transfer on the transcriptome of early equine conceptus membranes C. Gibson, M. de Ruijter-Villani, S. Bauersachs, T.A.E. Stout #72 - Oocytes from aged mares show reduced expression of mRNA for key spindle assembly checkpoint components - M. Rizzo, M. de Ruijter-Villani, C. Deelen, M. Beitsma, S. Cristarella, M. Quartuccio, T.A.E. Stout

#1 - Intrauterine administration of coconut oil to early pregnant mares increases embryonic vesicle size - Sandra Wilsher and WR Allen
#2 - Effect of Mesenchymal Stem Cells on Equine Ovarian Follicular Development - Sicilia T Grady, Ashlee E Watts, and Katrin Hinrichs
#3 - Is spontaneously double ovulation associate with incidence of EPL after nonsurgical embryo transfer in mares? - Hamed Farnia, N. Largel, S.M. Zuviria, J.I. Lopez Echenique
#4 - The Effect of Eight Antioxidants on Cryopreserved Equine Sperm Quality - Zhuangyuan Wu, Jingbo Chen, Yongming Luo, Xinbao Zheng, Hong Dong, Fei Huo
#5 - Comparison between open and closed vitrification system in equine collapsed embryos - Weiss, J. Blanco, M. Sanchez, R. Arbouin, C. Schockemöhle, P.
#7 - Expression of mesenchymal stem/stromal cell markers and steroid hormone receptors in the equine endometrium - Rink, E., Kuhl, J., Aurich, C., French, H., Nino-Fong, R., Watson, E., Donadeu, F.X.
#12 - Cooling equine embryo for 24h prior to vitrification: What are the consequences on its viability? - Maud Caillaud, Estelle Provost And Florence Guignot
#13 - Efficiency of two prostaglandin F2a analogs for synchronization of estrus and ovulation in mares - Juliane Kuhl, Jörg E. Aurich, Christine Aurich
#14 - Comparison of the efficacy of different single doses of buserelin with hCG for timed ovulation induction in the mare. - Newcombe, JR and Cuervo-Arango, J
#16 - Embryo Transfer and its influence on the Polo Argentino breed in the last 25 years Martinez - Zuviria S. and Zagrajczuk A.
#18 - The influences of age, laminitis and serum ACTH concentration on embryo recovery from Arabian mares - Jenny Katrine Boye, Valentina Barone and JG Oriol
#20 - Thawed stallion sperm refrozen without cryoprotectants activates cow oocytes after intracytoplasmic sperm injection (ICSI) - Jose Len, Dave Kleeman, Jen Kelly
#21 - Application of flunixin meglumine at the time of embryo transfer and the effect on the fertility - Okada, C.T.C., Teixeira Martini Segabinazi, L.G., Alvarenga, M.A.
#26 - Assessment of fetal sex determination in early equine pregnancy by isolation of fetal cells from the maternal circulation - Martin Köhne, Michaela Prchal-Murphy, Martin Hofer, Ralf Steinborn, Christine Aurich
#28 - Attempts to improve synchronization of ovulation and induce superovulation in the mare - Shuaishuai Wu, Nan Li, Shumin Wang, Guocai Han, and Shenming Zeng
#29 - Ultrasonographic evaluation of fetal growth between full siblings Arabian foals gestating in their genetic dams and embryo transfer recipients. Merridale-Punter M, Galinelli NC and Oriol JG
#35 - Proteomic analysis of the cervical mucus plug during pregnancy in mares - Loux, S.C., Scoggin, K., Troedsson, M. H., Squires, E.L., Ball, B.A.
#36 - Report of frozen equine embryos transfer in the Czech Republic - Josef Muller, Jindrich Muller
#37 - Influence of mare parity on weight, height, thoracic circumference and vitality of neonatal foals - FJ Affonso, MG Meirelles, MA Alonso, CF Guimaraes, KM Lemes, M Nichi, CB Fernandes
#38 - Effect of maternal age on estrogen production in mid to late pregnancy in the mare - MA Alonso, FJ Affonso, MG Meirelles, R Simas, DF Souza, VV Hernandes, M Nichi, MN Eberlin, CB Fernandes
#40 - Potential to reduce negative effects of exercise stress on number of pregnancies in a commercial embryo transfer program. - Pinto, M., Miragaya, M., Burns, P., Douglas, R. and Neild, D.
#41 - Can pregnancy survival after embryo transfer be predicted based on early ultrasound examinations in the recipient mare? - Morrissey JK, Ferris RA, Trundell D, Loncar K, Burden C, Scofield D, McCue PM
#44 - Influence of insemination on regulatory T cells in the peripheral blood of estrous mares - Constanze Hartmann, Wilhelm Gerner, Armin Saalmüller, Christine Aurich
#46 - Comparative efficacy of hCG or GnRH analogue (lecirelin acetate) on follicular dynamics, degree of endometrial edema, sexual behavior, ovulation and pregnancy rate in crossbred broodmares - Fayzan Sultan Awan, Muhammad Usman Mehmood, Abdul Sattar, Nasim Ahmad
#48 - Can we predict fertility in prepubertal female horses using Anti-Müllerian hormone? - Dragos Scarlet, Natascha Ille, Manuela Wulf, Ingrid Walter, Christine Aurich
#49 - The effect of exercise of the donor mare in an embryo transfer program - M. Christoffersen, M. Dige Soegaard, H. Gervi Pedersen and R. Munk Andersen
#51 - Effect of firocoxib on ovulation and fertility rates of embryo donor mares - Friso A M, Cyrino M A, Teoro M T, Alvarenga M A
#53 - In vitro growth of small equine embryos after vitrification - Celina M Checura and Alexis Powers
#54 - Preimplantation Gender Determination on Equine Embryos using LAMP - P. Dini, M. Van Poucke, C. Herrera, L. Peelman and Peter Daels
#55 - Comparison of pregnancy rates between acyclic mules and cyclic mares as recipients for embryo transfer - C.E. Camargo, R.C. Macan, S.F. Rechsteiner, E.L. Gastal
#57 - Gene expression prior and after vitrification of equine CCO’s - Polenz, M.F., Pereira, G.R., Augusto, C.G., Fiala Rechsteiner, S.
#61 - Holding Equine Oocytes in Syngro® Embryo Holding Medium at 4°C. - P. Dini, O. Bogado, M. Hostens and P. Daels
#64 - Improving frozen equine sperm motility by the addition of different concentrations of Coenzyme Q10 to a freezing extender - Lagares, MA, Luz, SB, Coutinho, IM, Morais, BM and Stahlberg, R
#66 - Transport of Equine Oocytes in Syngro® Embryo Holding Medium using a Passive Cooling System (Equitainer®) - Kaatje Danielle Ducheyne, Francisco Guaimas, Romina Santa Cruz, Julieta Mertian, Nicolás Matiás Ortega, Peter Daels and Adrián Mutto
#69 - Efficacy of a single injection of buserelin acetate in saline solution and a single injection of hCG for the induction of ovulation in mares - L. Normandin, C. Frilley, A. Cochard, H Terris, J.-F. Bruyas
#70 - Is there a repeatability of the size of follicles at the ovulation within individuals in mares - C. Lefrançois, J.-F. Bruyas
#71 - Effect of asynchronous embryo transfer on the transcriptome of early equine conceptus membranes C. Gibson, M. de Ruijter-Villani, S. Bauersachs, T.A.E. Stout
#72 - Oocytes from aged mares show reduced expression of mRNA for key spindle assembly checkpoint components - M. Rizzo, M. de Ruijter-Villani, C. Deelen, M. Beitsma, S. Cristarella, M. Quartuccio, T.A.E. Stout

Monday, July 4 - Day 3

We will propose a choice of organized visits that delegates can book before or at the conference (fee-based). Guided tours through the old part of town; boat ride through the city center; visit of the veterinary hospital and anatomy museum; etc.

Keynote Speakers

	The Horse Catalyzed Birth of Modern Veterinary Medicine in 18th Century France Peter Bols - University of Antwerp, Faculty of Biomedical, Pharmaceutical and Veterinary Sciences, Department of Veterinary Sciences, Laboratory of Veteinary Physiology, Wilrijk, Belgium Available online in the Journal of Equine Veterinary Science
	Gametes, Embryos and their Epigenome: Considerations for Equine Embryo Technologies Pascale Chavatte - Institut National de Recherche Agronomique INRA, Biologie du Développement et Reproduction, Domaine de Vilvert, Jouy en Josas, France Available online in the Journal of Equine Veterinary Science
	Clinical Applications of Preimplantation Genetic Testing in Equine, Bovine and Human Embryos Carolina Hererra - Clinic for Reproductive Medicine, Vetsuisse - Faculty, University of Zurich, Switzerland and Institut Suisse de Médecine Equine (ISME), Agroscope, Universität Bern, Switzerland. Available online in the Journal of Equine Veterinary Science
	Maternal Recognition of Pregnancy in the Context of Equine Embryo Transfer Claudia Klein - Faculty of Veterinary Medicine, University of Calgary, Canada. Available online in the Journal of Equine Veterinary Science
	In vitro Production of Equine Embryos and Cloning: Today’s Status Marc P. Maserati Jr - InVitroBrasil Clonagem Animal, Fazenda São Francisco, SP 340; Km 166, Caixa Postal 238, Mogi Mirim, SP Brasil. Available online in the Journal of Equine Veterinary Science
	In vitro Production of Equine Embryos and Cloning: Today’s Status Adrian Mutto - Laboratorio de Desarrollos Biotecnológicos, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús(IIB-INTECH), Provincia de Buenos Aires, Argentina. Available online in the Journal of Equine Veterinary Science
	Cryopreservation of Equine Embryos Edward L. Squires - Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA. Available online in the Journal of Equine Veterinary Science
	Genome Editing in Large Animals James West - Department of Medicine, Vanderbilt University Medical Center, Nashville, TN and AgGenetics, Nashville, TN, USA. Available online in the Journal of Equine Veterinary Science

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